PXD037530

PXD037530 is an original dataset announced via ProteomeXchange.

Title	Protein Lactylation modification and Proteomics Features in Cirrhosis Patients after MSCs Treatment.
Description	Mesenchymal stem cell (MSC) therapy improves liver function in patients with liver cirrhosis. However, the therapeutic mechanism underlying MSC therapy remains unclear. Therefore, this study aimed to elucidate the therapeutic mechanism underlying MSC therapy by analyzing changes in the modification and expression of proteins 1-month post-treatment with MSCs. This prospective study included 11 patients with cirrhosis who received MSC injection in the First Hospital of Lanzhou University. The laboratory indexes before and after treatment were collected to evaluate the clinical treatment effect of MSCs, and peptide segments were extracted from liver tissue before and after treatment to revealed the protein expression and lactylation modification. Meanwhile, weighted gene co-expression network analysis was used to analyze co-expression protein modules and their relationship with clinical features. The patients with liver cirrhosis showed an improvement trend after receiving MSC treatment, specifically, the liver protein synthesis function was significantly improved and coagulation function was also significantly improved. Proteomics combined with lactic acid proteomics revealed 160 Lysine lactylation (Kla) sites of 119 proteins. The downregulated lactylated proteins were associated with metabolic processes. The upregulated lactylated proteins were involved in biological processes. The protein-based co-expression module was significantly related to alkaline phosphatase, total bilirubin (TBIL), indirect bilirubin (IBIL), direct bilirubin (DBIL), and ALB, and 10 proteins significantly related to ALB and 10 proteins significantly related to bilirubin were screened. The changes in clinical indexes before and after treatment indicate that the clinical effects of MSC treatment are related to cell metabolism. Overall, this study is the first study to comprehensively and systematically reveal the changes of lactylated proteins and expression after treatment with MSC by the self-control of patients, and lays a foundation for understanding the functions of lactylation modification in MSC therapy.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:12:07.849.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ye xie
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-18 08:03:27	ID requested
⏵ 1	2024-01-26 07:12:08	announced

Xie Y, Li Y, Yao J, Song X, Wang H, Zhang J, Li X, Protein Lactylation Modification and Proteomics Features in Cirrhosis Patients after UC-MSC Treatment. Curr Issues Mol Biol, 45(10):8444-8460(2023) [pubmed]

10.3390/cimb45100532;

submitter keyword: liver cirrhosis

Mesenchymal stem cell

cell therapy

lactylation modification

proteomics analysis.

Xun Li
contact affiliation	The First Clinical Medical College, Lanzhou University; Key Laboratory of Biotherapy and Regenerative Medicine of Gansu Province; The Department of General Surgery, The First Hospital of Lanzhou University; Hepatopancreatobiliary Surgery Institute of Gansu Province, Lanzhou 730000, Gansu Province, China.
contact email	lxdr21@126.com
lab head
ye xie
contact affiliation	The First Clinical Medical College, Lanzhou University
contact email	xiey19@lzu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037530
     1. Label: PRIDE project
     2. Name: Protein Lactylation modification and Proteomics Features in Cirrhosis Patients after MSCs Treatment.