PXD037520

PXD037520 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of outer membrane vesicles from Fusobacterium nucleatum in the cancer environment
Description	At present, the function of Fusobacterium nucleosocci outer membrane vesicles under normal intestinal environmental pH conditions has been mainly studied, but there are few studies related to the function of Fusobacterium nucleosius outer membrane vesicles under cancer microenvironment pH conditions. The purpose of this study was to pioneer the comparative analysis of the composition of Fusobacterium nucleosibacterium outer membrane vesicle proteins under normal intestinal environmental pH conditions and cancer microenvironment pH conditions, and to find out the virulence factor protein of Fusobacterium nucleosocci outer membrane vesicles under cancer microenvironment pH conditions. To do this, we adjusted the initial pH of TSPC medium to around 6.0 using acidic cysteine to mimic the cancer microenvironment pH conditions. We combined ultracentrifugation and density gradient centrifugation to separate and extract Fusobacterium nucleosi's outer membrane vesicles from two pH environments, and used TMT quantitative proteomics to identify proteins in outer membrane vesicles. A total of 991 proteins were identified, compared with normal outer membrane vesicles, 306 proteins in the outer membrane vesicles in the tumor environment were up-regulated and 360 proteins were down-regulated, and some of the up-regulated proteins of the out-membrane vesicles in the tumor environment were virulence factor proteins, such as multiple pentatype autotransporters and MORN2 domain proteins, and the functions of these proteins were mainly related to adhesion and invasion. In addition, the down-regulation of enzyme levels involved in butyrate metabolism in the outer membrane vesicles of Fusobacterium nucleosionae under the condition of cancer microenvironment pH inhibits the butyric acid production capacity of Fusobacterium nucleobacteria, resulting in a decrease in butyric acid production capacity, which may promote the development of colorectal cancer. In conclusion, quantitative proteomics comparative analysis has increased our understanding of Fusobacterium nucleosom outer membrane vesicle virulence protein in the tumor environment.
HostingRepository	iProX
AnnounceDate	2022-10-18
AnnouncementXML	Submission_2023-08-29_21:51:36.799.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuxin Wang
SpeciesList	scientific name: Fusobacterium nucleatum subsp. vincentii; NCBI TaxID: 155615;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 22:53:35	ID requested
1	2023-04-17 21:37:28	announced
2	2023-04-17 23:43:48	announced	2023-04-18: Update information.
⏵ 3	2023-08-29 21:51:37	announced	2023-04-18: Update information. 2023-08-30: Update publication information.

Zhang X, Wang Y, Fan R, Zhang L, Li Z, Zhang Y, Zheng W, Wang L, Liu B, Quan C, Quantitative Proteomic Analysis of Outer Membrane Vesicles from Fusobacterium nucleatum Cultivated in the Mimic Cancer Environment. Microbiol Spectr, 11(4):e0039423(2023) [pubmed]

submitter keyword: outer membrane vesicles, Fusobacterium nucleatum

Chunshan Quan
contact affiliation	Dalian Minzu University
contact email	mikyeken@dlnu.edu.com
lab head
Yuxin Wang
contact affiliation	Dalian Minzu University
contact email	wangyuxinby@163.com
dataset submitter

iProX dataset URI