PXD037519

PXD037519 is an original dataset announced via ProteomeXchange.

Title	Multi-omics analysis reveals metabolic pathways of benzene biodegradation under sole or mixed carbon sources
Description	Proteomics and metabolomics can be used to efficiently and conveniently assess proteins and metabolites during microbial degradation of pollutants; these analytical techniques are closely correlated and mutually confirmed and provide a means to explore degradation pathways. The degradation pathways and regulatory characteristics of Microbacterium sp. M.CSW3 for the metabolism of benzopyrene (BaP) alone and in the presence of phenanthrene and pyrene were investigated using these omics. Proteomics results showed that CLP protease, ABC transporter, aldehyde dehydrogenase, and enoyl-CoA hydratase were important for the metabolism of polycyclic aromatic hydrocarbons (PAHs). Five pathways for the microbial degradation of benzopyrene were suggested by metabolomics, and phenanthrene was identified as an intermediate metabolite. Further, correlation analysis showed that the two stress environments inhibited the biosynthesis of bacterial amino acids. The glycolysis/gluconeogenesis and fatty acid degradation pathways played a positive role in energy acquisition and PAHs degradation. Addition of low-molecular-weight PAHs promoted the production of related enzyme systems and also make the degradation of BaP more thorough. This study provides efficient concepts and methods for the exploration of degradation mechanisms of high-molecular-weight PAHs and co-metabolism of mixed PAHs.
HostingRepository	iProX
AnnounceDate	2022-10-17
AnnouncementXML	Submission_2022-10-17_22:51:49.123.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Man Hu
SpeciesList	scientific name: Microbacterium; NCBI TaxID: 33882;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 22:51:22	ID requested
⏵ 1	2022-10-17 22:51:49	announced

Dataset with its publication pending

submitter keyword: benzopyrene, proteomics, metabolomics, Microbacterium sp., polycyclic aromatic hydrocarbons, co-metabolism

Yi Zhu
contact affiliation	Bejing Normal University
contact email	zhuyi@bnu.edu.cn
lab head
Man Hu
contact affiliation	Bejing Normal University
contact email	humanxx123@163.com
dataset submitter

iProX dataset URI