PXD037518
PXD037518 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic response of S. aureus to alpha-mangostin |
| Description | S. aureus isolate was inoculated and cultured to exponential growth phase (OD600 at 0.5) in TSB and divided into two groups, the control group and the drug treated group. alpha-mangostin was added to the drug treated group until a final concentration of 1/2× MIC reached, and DMSO added in the control group. Then, cultures in both groups were cultivated for 2h at 180rpm. The microbial was centrifuged at 12000rpm for 10min, washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer. |
| HostingRepository | iProX |
| AnnounceDate | 2022-10-17 |
| AnnouncementXML | Submission_2023-06-25_20:06:11.861.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zewen Wen |
| SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
| ModificationList | methionine oxidation with neutral loss of 64 Da |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-10-17 22:44:59 | ID requested | |
| 1 | 2022-10-17 22:45:21 | announced | |
| ⏵ 2 | 2023-06-25 20:06:12 | announced | 2023-06-26: Update publication information. |
Publication List
| Deng X, Xu H, Li D, Chen J, Yu Z, Deng Q, Li P, Zheng J, Zhang H, . Pol J Microbiol, 72(2):199-208(2023) [pubmed] |
Keyword List
| submitter keyword: Staphylococcus aureus, alpha-mangostin |
Contact List
| Zewen Wen | |
|---|---|
| contact affiliation | Huazhong University of Science and Technology Union Shenzhen Hospital |
| contact email | wenzw05@163.com |
| lab head | |
| Zewen Wen | |
| contact affiliation | Huazhong University of Science and Technology Union Shenzhen Hospital |
| contact email | wenzw05@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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