PXD037513 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ki-67 is necessary during DNA replication for forks protection and genome stability |
Description | Ki-67 molecular functions and properties have remained quite obscure for a long time, despite its importance as a major proliferation marker in clinic. Only recently it has been shown that Ki-67 has a major role in the formation of the mitotic chromosome periphery compartment, and it is a protein phosphatase 1 (PP1) binding protein. Understanding Ki-67 functions at different stages of the cell cycle has been so far hindered by the fact that the different phenotypes observed in cells upon Ki-67 depletion could be the outcome of secondary effects. Here, by using Auxin-inducible-degron (AID) system, we show that Ki-67 degradation at the G1/S boundary causes the disassembly of the replication machinery and leads to DNA damage. This triggers the interferon response and causes replication delay. Our results support the model whereby Ki-67 contributes to replication forks protection, and it is important for timely DNA replication and genome maintenance. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:35:57.940.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paola Vagnarelli |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos; Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-17 07:14:22 | ID requested | |
1 | 2024-04-13 11:45:40 | announced | |
⏵ 2 | 2024-10-22 06:35:58 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Ki-67, SILAC, genome stability, Cell cycle, DNA replication |
Contact List
Paola Vagnarelli |
contact affiliation | Brunel University London |
contact email | paola.vagnarelli@brunel.ac.uk |
lab head | |
Paola Vagnarelli |
contact affiliation | Brunel University London |
contact email | Paola.Vagnarelli@brunel.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037513
- Label: PRIDE project
- Name: Ki-67 is necessary during DNA replication for forks protection and genome stability