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PXD037513

PXD037513 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleKi-67 is necessary during DNA replication for forks protection and genome stability
DescriptionKi-67 molecular functions and properties have remained quite obscure for a long time, despite its importance as a major proliferation marker in clinic. Only recently it has been shown that Ki-67 has a major role in the formation of the mitotic chromosome periphery compartment, and it is a protein phosphatase 1 (PP1) binding protein. Understanding Ki-67 functions at different stages of the cell cycle has been so far hindered by the fact that the different phenotypes observed in cells upon Ki-67 depletion could be the outcome of secondary effects. Here, by using Auxin-inducible-degron (AID) system, we show that Ki-67 degradation at the G1/S boundary causes the disassembly of the replication machinery and leads to DNA damage. This triggers the interferon response and causes replication delay. Our results support the model whereby Ki-67 contributes to replication forks protection, and it is important for timely DNA replication and genome maintenance.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:35:57.940.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPaola Vagnarelli
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion Lumos; Orbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-17 07:14:22ID requested
12024-04-13 11:45:40announced
22024-10-22 06:35:58announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Ki-67, SILAC, genome stability, Cell cycle, DNA replication
Contact List
Paola Vagnarelli
contact affiliationBrunel University London
contact emailpaola.vagnarelli@brunel.ac.uk
lab head
Paola Vagnarelli
contact affiliationBrunel University London
contact emailPaola.Vagnarelli@brunel.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
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