PXD037513

PXD037513 is an original dataset announced via ProteomeXchange.

Title	Ki-67 is necessary during DNA replication for forks protection and genome stability
Description	Ki-67 molecular functions and properties have remained quite obscure for a long time, despite its importance as a major proliferation marker in clinic. Only recently it has been shown that Ki-67 has a major role in the formation of the mitotic chromosome periphery compartment, and it is a protein phosphatase 1 (PP1) binding protein. Understanding Ki-67 functions at different stages of the cell cycle has been so far hindered by the fact that the different phenotypes observed in cells upon Ki-67 depletion could be the outcome of secondary effects. Here, by using Auxin-inducible-degron (AID) system, we show that Ki-67 degradation at the G1/S boundary causes the disassembly of the replication machinery and leads to DNA damage. This triggers the interferon response and causes replication delay. Our results support the model whereby Ki-67 contributes to replication forks protection, and it is important for timely DNA replication and genome maintenance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:35:57.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paola Vagnarelli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 07:14:22	ID requested
1	2024-04-13 11:45:40	announced
⏵ 2	2024-10-22 06:35:58	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Ki-67, SILAC, genome stability, Cell cycle, DNA replication

Paola Vagnarelli
contact affiliation	Brunel University London
contact email	paola.vagnarelli@brunel.ac.uk
lab head
Paola Vagnarelli
contact affiliation	Brunel University London
contact email	Paola.Vagnarelli@brunel.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD037513

PRIDE project URI

• PRIDE
  1. PXD037513
     1. Label: PRIDE project
     2. Name: Ki-67 is necessary during DNA replication for forks protection and genome stability