PXD037495

PXD037495 is an original dataset announced via ProteomeXchange.

Title	The effect of GLP-1 receptor agonist liraglutide on the rat neurointermediate lobe phosphoproteome
Description	Obesity and its co-morbidities, such as diabetes and hypertension, can significantly reduce a person’s quality of life and place huge pressure on healthcare resources. When we eat a meal our gut and brain release hormones to control the amount of food and fluid we ingest to prevent overeating. One of these hormones is called glucagon-like peptide 1 (GLP-1) and is released from intestinal cells in response to food intake, but also produced and released in the brain. Drug analogues of GLP-1 are already in use in the clinic to treat both diabetes and obesity. The aim of this work was to obtain fundamental knowledge about a GLP-1 receptor population in nerve terminals of the posterior pituitary gland. We have investigated the pharmacological actions of GLP-1 using a selective receptor agonist called liraglutide, a drug that is approved for diabetes and obesity treatment in humans. Our work has focussed on the phosphoproteome of the neurointermediate lobe (posterior pituitary + intermediate lobe) of the rat pituitary gland 30 minutes after intraperitoneal injection of liraglutide (100 µg/kg) compared to vehicle controls (n = 6 animals per group). New understanding of this GLP-1 receptor population is essential for our knowledge of current treatments of diabetes and obesity that use stable peptide analogues in humans.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:20.947.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Greenwood
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 01:38:17	ID requested
1	2023-03-01 23:32:28	announced
⏵ 2	2023-11-14 08:58:21	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Phosphoproteomics, LC-MSMS, Liraglutide, Posterior pituitary,Pituitary gland, Intermediate lobe, Phosphorylation

David Murphy
contact affiliation	Molecular Neuroendocrinology Research Group, Bristol Medical School: Translational Health Sciences, University of Bristol, Dorothy Hodgkin Building, Bristol, United Kingdom
contact email	d.murphy@bristol.ac.uk
lab head
Michael Greenwood
contact affiliation	University of Bristol
contact email	Mike.Greenwood@bristol.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037495
     1. Label: PRIDE project
     2. Name: The effect of GLP-1 receptor agonist liraglutide on the rat neurointermediate lobe phosphoproteome