PXD037479

PXD037479 is an original dataset announced via ProteomeXchange.

Title	RB1 mass spec with or without MYC amplification
Description	293T cells were transfected with Flag-tagged RB1 with or without MYC. After 48 h, the cells were lysed with IP buffer and immunoprecipitated with Flag-conjugated agarose beads (Sigma-Aldrich). The bound proteins were eluted with 2% SDS and digested overnight with sequencing grade modified trypsin (Promega, PRV5111). The obtained peptides were analyzed using a nanoflow EASY-nLC 1200 system (Thermo Fisher Scientific, Odense, Denmark) coupled to an Orbitrap Exploris480 mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). The results were processed with the UniProt human protein database (75,004 entries, download on 07-01-2020) using Protein Discoverer (Version 2.4.1.15, Thermo Fisher Scientific) and Mascot (Version 2.7.0, Matrix Science).
HostingRepository	iProX
AnnounceDate	2022-10-17
AnnouncementXML	Submission_2024-03-07_19:19:20.131.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jian Ma
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 01:18:22	ID requested
1	2022-10-17 01:18:39	announced
⏵ 2	2024-03-07 19:19:21	announced	2024-03-08: Update information.

Ma J, Li L, Ma B, Liu T, Wang Z, Ye Q, Peng Y, Wang B, Chen Y, Xu S, Wang K, Dang F, Wang X, Zeng Z, Jian Y, Ren Z, Fan Y, Li X, Liu J, Gao Y, Wei W, Li L, MYC induces CDK4/6 inhibitors resistance by promoting pRB1 degradation. Nat Commun, 15(1):1871(2024) [pubmed]

submitter keyword: IgG, RB1, RB1-MYC

Jian Ma
contact affiliation	Xi'an Jiaotong University
contact email	majian0922@gmail.com
lab head
Jian Ma
contact affiliation	Xi'an Jiaotong University
contact email	majian0922@gmail.com
dataset submitter

iProX dataset URI