PXD037450

PXD037450 is an original dataset announced via ProteomeXchange.

Title	Integrated glycomic and proteomic signatures of butyrate-stimulated colorectal cancer cell line differentiation
Description	It was previously observed that changes in the expression of specific proteins as well as protein glycosylation occur with differentiation. In this study, specific mucin O-glycans were identified that mark butyrate-induced epithelial differentiation of the intestinal cell line CaCo-2, by applying porous graphitized carbon nano-liquid chromatography with electrospray ionization tandem mass spectrometry (PGC-nanoLC-MS/MS). Moreover, a quantitative proteomic approach was used to decipher changes in the cell proteome. By performing an integrative approach, we generated hypotheses about glycosylation signatures of specific cell adhesion proteins.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:18.582.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katarina Madunic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-14 14:45:01	ID requested
1	2023-05-10 14:01:36	announced
⏵ 2	2023-11-14 08:47:27	announced	2023-11-14: Updated project metadata.

Maduni, ć K, Luijkx YMCA, Mayboroda OA, Janssen GMC, van Veelen PA, Strijbis K, Wennekes T, Lageveen-Kammeijer GSM, Wuhrer M, O-Glycomic and Proteomic Signatures of Spontaneous and Butyrate-Stimulated Colorectal Cancer Cell Line Differentiation. Mol Cell Proteomics, 22(3):100501(2023) [pubmed]

submitter keyword: Caco-2, differentiation

Manfred Wuhrer
contact affiliation	CPM, Leiden University Medical Center
contact email	M.Wuhrer@lumc.nl
lab head
Katarina Madunic
contact affiliation	University of Copenhagen
contact email	littlesister89st@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD037450

PRIDE project URI

• PRIDE
  1. PXD037450
     1. Label: PRIDE project
     2. Name: Integrated glycomic and proteomic signatures of butyrate-stimulated colorectal cancer cell line differentiation