PXD037443

PXD037443 is an original dataset announced via ProteomeXchange.

Title	Exploring Alternative Cysteine-reactive Chemistry for MS-cleavable Cross-linking Mass Spectrometry
Description	To enable global PPI mapping, we have explored an alternative cysteine labeling chemistry, and thus designed and synthesized a sulfoxide-containing MS-cleavable haloacetamide-based cross-linker, DiBromoAcetamide SulfOxide (DBrASO). In combination with a newly developed SEC-HpHt fractionation method, we have successfully applied DBrASO for cross-linking of HEK293 cell lysates and demonstrated its capability to complement lysine-reactive reagents in order to expand PPI coverage at systems-level.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:00.689.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fenglong Jiao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-14 07:17:03	ID requested
1	2023-07-20 11:08:14	announced
⏵ 2	2023-11-14 09:03:02	announced	2023-11-14: Updated project metadata.

Jiao F, Salituro LJ, Yu C, Gutierrez CB, Rychnovsky SD, Huang L, Exploring an Alternative Cysteine-Reactive Chemistry to Enable Proteome-Wide PPI Analysis by Cross-Linking Mass Spectrometry. Anal Chem, 95(4):2532-2539(2023) [pubmed]

submitter keyword: cysteine, proteomics,cross-linking mass spectrometry

Lan Huang
contact affiliation	University of California, Irvine
contact email	lanhuang@uci.edu
lab head
Fenglong Jiao
contact affiliation	University of California, Irvine
contact email	fenglonj@uci.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD037443

PRIDE project URI

• PRIDE
  1. PXD037443
     1. Label: PRIDE project
     2. Name: Exploring Alternative Cysteine-reactive Chemistry for MS-cleavable Cross-linking Mass Spectrometry