PXD037439

PXD037439 is an original dataset announced via ProteomeXchange.

Title	Proteomics reveals how the tardigrade damage suppressor protein teaches transfected human cells to survive UV-C stress
Description	The genome sequencing of the tardigrade Ramazzottius varieornatus revealed a unique nucleo-some-binding protein, named Damage Suppressor (Dsup), which resulted to be crucial for the extraordinary abilities of tardigrades in surviving extreme stresses, such as UV. Evidence in Dsup transfected human cells suggests that Dsup mediates an overall response of DNA damage signaling, DNA repair and cell cycle regulation resulting in an acquired resistance to stress. Given these promising outcomes, our study attempts to provide a wider comprehension of the molecular mechanisms modulated by Dsup in human cells, and to explore the Dsup-activated molecular pathways under stress. We performed a differential proteomic analysis of Dsup-transfected and control human cells, under basal condition and at 24-hour recovery after exposure to UV-C. We demonstrate by enrichment and network analyses, for the first time, that even in the absence of external stimuli and more significantly after stress, Dsup activates mech-anisms involved with the Unfolded Protein Response, the mRNA processing and stability, cyto-plasmic stress granules, the DNA Damage Response and the telomere maintenance. In conclu-sion, our results shed new light on Dsup-mediated protective mechanisms, and increase our knowledge of the molecular machineries of extraordinary protection against UV-C stress.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:04:28.304.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enxhi Shaba
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-14 07:15:49	ID requested
1	2023-07-31 01:36:35	announced
⏵ 2	2023-11-14 09:04:29	announced	2023-11-14: Updated project metadata.

Shaba E, Landi C, Marzocchi C, Vantaggiato L, Bini L, Ricci C, Cantara S, Proteomics Reveals How the Tardigrade Damage Suppressor Protein Teaches Transfected Human Cells to Survive UV-C Stress. Int J Mol Sci, 24(14):(2023) [pubmed]

submitter keyword: proteomics, stress granules,Dsup, telomere lenght, DNA damage response

Prof. Luca
contact affiliation	Functional Proteomics Lab, Department of Life Sciences, University of Siena, 53100 Siena, Italy
contact email	luca.bini@unisi.it
lab head
Enxhi Shaba
contact affiliation	University of Siena
contact email	shaba3@student.unisi.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD037439

PRIDE project URI

• PRIDE
  1. PXD037439
     1. Label: PRIDE project
     2. Name: Proteomics reveals how the tardigrade damage suppressor protein teaches transfected human cells to survive UV-C stress