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PXD037418

PXD037418 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe antimicrobial peptide Magainin-2 interacts with BamA impairing folding of E. coli membrane proteins
DescriptionIn this paper we investigated the mechanism of action of Maganin-2 (Mag-2), a well-known antimicrobial peptide isolated from the African clawed frog Xenopus laevis, by functional proteomic approaches. Several proteins belonging to E. coli macromolecular membrane complexes were identified as Mag-2 putative interactors. Among these, we focused our attention on BamA a membrane protein belonging to the BAM complex responsible for the folding and insertion of nascent β‐barrel Outer Membrane Proteins (OMPs) in the outer membrane. In silico predictions by molecular modelling and in vitro fluorescence binding and Light Scattering experiments carried out using a recombinant form of BamA confirmed the formation of a stable Mag-2/BamA complex and indicated a high affinity of the peptide for BamA. The functional implications of these interactions were investigated by two alternative and complementary approaches. The number of outer membrane proteins OmpA and OmpF produced in E. coli following Mag-2 incubation was evaluated by both western blot analysis and quantitative tandem mass spectrometry in MRM scan mode. In both experiments, a gradual decrease in outer membrane protein production with time was observed because of Mag-2 treatment.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:42:46.317.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGabriella Pinto
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-14 04:47:05ID requested
12023-03-11 04:41:07announced
22023-11-14 08:42:54announced2023-11-14: Updated project metadata.
Publication List
Di Somma A, Can, é C, Moretta A, Illiano A, Pinto G, Cavasso D, Amoresano A, Paduano L, Duilio A, membrane proteins. Front Chem, 10():1013788(2022) [pubmed]
Keyword List
submitter keyword: proteomics, mechanism of action, structural and functional characterization,Antimicrobial peptides, membrane proteins
Contact List
Prof. Angela Amoresano
contact affiliationDepartment of Chemical Science, University of Naples 'Federico II'
contact emailangamor@unina.it
lab head
Gabriella Pinto
contact affiliationDepartment of chemical science University of Naples
contact emailgabriella.pinto@unina.it
dataset submitter
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Dataset FTP location
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