PXD037416

PXD037416 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative analysis of protein ubiquitination
Description	Protein ubiquitination orchestrates nearly all eukaryotic cellular events.1 It starts by attaching ubiquitin through isopeptide bonds to a single or multiple lysine residues of a target protein via a coordinated enzymatic reaction involving activating (E1), conjugating (E2), and ligating (E3) enzymes to form mono- or multi-mono-ubiquitinated products. There is a need for a technique that can facilitate the high-yield production of monoubiquitinated proteins. Here we present an efficient approach that fills this gap.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:49.084.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yue Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-14 04:46:20	ID requested
1	2023-05-10 13:40:53	announced
⏵ 2	2023-11-14 08:46:54	announced	2023-11-14: Updated project metadata.

Publication List

Nelson SL, Li Y, Chen Y, Deshmukh L, Avidity-Based Method for the Efficient Generation of Monoubiquitinated Recombinant Proteins. J Am Chem Soc, 145(14):7748-7752(2023) [pubmed]

Nelson SL, Li Y, Chen Y, Deshmukh L, Avidity-Based Method for the Efficient Generation of Monoubiquitinated Recombinant Proteins. J Am Chem Soc, 145(14):7748-7752(2023) [pubmed]

Keyword List

submitter keyword: IBAQ-Ub,ubiquitination, stoichiometry, LCMS, quantitative proteomics

submitter keyword: IBAQ-Ub,ubiquitination, stoichiometry, LCMS, quantitative proteomics

Contact List

Yue Chen
contact affiliation	University of Minnesota
contact email	yuechen@umn.edu
lab head
Yue Chen
contact affiliation	University of Minnesota
contact email	ychen2001@yahoo.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD037416
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD037416

PRIDE project URI

Repository Record List

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