PXD037415

PXD037415 is an original dataset announced via ProteomeXchange.

Title	Large-scale and Site-specific Mapping of Intact O-glycans on Glycopeptides with IMPa
Description	We employed the newly available IMPa O-glycoprotease from Pseudomonas aeruginosa for O-glycoproteomics analysis of cultured cells and tissues. The glycopeptides were extracted, purified, and conjugated to a solid support before an enzymatic cleavage by IMPa. O-glycopeptides were analyzed by EThcD, which allowed for a site-specific and global analysis of O-glycans, especially those sialylated and the Tn antigen. Through wild-type or SimpleCell HEK293 cells, we present two approaches, one for the analysis of total O-glycoproteome and the other for the Tn glycoproteome, and showed that IMPa and EThcD are necessary for confident localization of O-glycans on glycopeptides. We applied this method to study the O-glycoproteome of mouse brain, which revealed the high frequency of sialylated O-glycans and the Tn antigen on brain glycoproteins.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:37:34.065.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suttipong Suttapitugsakul
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-14 04:45:58	ID requested
1	2023-08-28 11:33:19	announced
⏵ 2	2023-11-14 07:37:34	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: O-glycoproteomics

IMPa

VVA

RAX

Tn antigen

Richard D. Cummings
contact affiliation	National Center for Functional Glycomics, Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School
contact email	rcummin1@bidmc.harvard.edu
lab head
Suttipong Suttapitugsakul
contact affiliation	Beth Israel Deaconess Medical Center and Harvard Medical School
contact email	ssuttapi@bidmc.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037415
     1. Label: PRIDE project
     2. Name: Large-scale and Site-specific Mapping of Intact O-glycans on Glycopeptides with IMPa