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PXD037415

PXD037415 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLarge-scale and Site-specific Mapping of Intact O-glycans on Glycopeptides with IMPa
DescriptionWe employed the newly available IMPa O-glycoprotease from Pseudomonas aeruginosa for O-glycoproteomics analysis of cultured cells and tissues. The glycopeptides were extracted, purified, and conjugated to a solid support before an enzymatic cleavage by IMPa. O-glycopeptides were analyzed by EThcD, which allowed for a site-specific and global analysis of O-glycans, especially those sialylated and the Tn antigen. Through wild-type or SimpleCell HEK293 cells, we present two approaches, one for the analysis of total O-glycoproteome and the other for the Tn glycoproteome, and showed that IMPa and EThcD are necessary for confident localization of O-glycans on glycopeptides. We applied this method to study the O-glycoproteome of mouse brain, which revealed the high frequency of sialylated O-glycans and the Tn antigen on brain glycoproteins.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:37:34.065.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSuttipong Suttapitugsakul
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-14 04:45:58ID requested
12023-08-28 11:33:19announced
22023-11-14 07:37:34announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: O-glycoproteomics
IMPa
VVA
RAX
Tn antigen
Contact List
Richard D. Cummings
contact affiliationNational Center for Functional Glycomics, Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School
contact emailrcummin1@bidmc.harvard.edu
lab head
Suttipong Suttapitugsakul
contact affiliationBeth Israel Deaconess Medical Center and Harvard Medical School
contact emailssuttapi@bidmc.harvard.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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