PXD037415 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Large-scale and Site-specific Mapping of Intact O-glycans on Glycopeptides with IMPa |
Description | We employed the newly available IMPa O-glycoprotease from Pseudomonas aeruginosa for O-glycoproteomics analysis of cultured cells and tissues. The glycopeptides were extracted, purified, and conjugated to a solid support before an enzymatic cleavage by IMPa. O-glycopeptides were analyzed by EThcD, which allowed for a site-specific and global analysis of O-glycans, especially those sialylated and the Tn antigen. Through wild-type or SimpleCell HEK293 cells, we present two approaches, one for the analysis of total O-glycoproteome and the other for the Tn glycoproteome, and showed that IMPa and EThcD are necessary for confident localization of O-glycans on glycopeptides. We applied this method to study the O-glycoproteome of mouse brain, which revealed the high frequency of sialylated O-glycans and the Tn antigen on brain glycoproteins. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:37:34.065.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Suttipong Suttapitugsakul |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-14 04:45:58 | ID requested | |
1 | 2023-08-28 11:33:19 | announced | |
⏵ 2 | 2023-11-14 07:37:34 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: O-glycoproteomics |
IMPa |
VVA |
RAX |
Tn antigen |
Contact List
Richard D. Cummings |
contact affiliation | National Center for Functional Glycomics, Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School |
contact email | rcummin1@bidmc.harvard.edu |
lab head | |
Suttipong Suttapitugsakul |
contact affiliation | Beth Israel Deaconess Medical Center and Harvard Medical School |
contact email | ssuttapi@bidmc.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037415
- Label: PRIDE project
- Name: Large-scale and Site-specific Mapping of Intact O-glycans on Glycopeptides with IMPa