PXD037407

PXD037407 is an original dataset announced via ProteomeXchange.

Title	Proteomics of mouse testis after cranial irradiation by tandem mass spectrometry
Description	Healthy adult male C57BL/6 mice (8 weeks of age) were randomly divided into sham irradiation group (sham) and cranial ir-radiation group (5 Gy  4 d). For the 5 Gy  4 d group, the head of mice were received 20 Gy X-rays (RAD Source RS 2000 series, Suwanee, USA), applied as four doses of 5 Gy in consecutive 4 days at a dose rate of 2.33 Gy/ min. The rest of mouse’s body was completely protected by a 2-cm thick lead shield. For the sham group, the mice were maintained in the same procedure except X-ray irradiation. No radiation leakage through the shield occurred in the present study, as verified by dose measurement of testis under shielding and morphological structure of peripheral main organs. Meanwhile, Monte Carlo simulation was used to calculate the absorbed dose of the peripheral main organs when mice head was irradiated with 20 Gy X-rays. The results showed that the absorbed dose of testis under shielding was 0.018 Gy, which was only 0.9‰ of cranial absorbed dose. Tandem mass tag (TMT) quantitative proteomic analysis of testis at 4 W after cranial irradiation was carried out by LC-BIO Co., Ltd (Hangzhou, China). The differentially expressed proteins (DEPs) were screened and obtained using the cutoffs of fold change > 1.2 and P < 0.05. For bioinformatics analysis, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed using the DAVID 6.8 database (https://david.ncifcrf.gov/).
HostingRepository	iProX
AnnounceDate	2022-10-14
AnnouncementXML	Submission_2022-10-13_19:39:01.752.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ling Guo
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-13 19:38:45	ID requested
⏵ 1	2022-10-13 19:39:02	announced

Dataset with its publication pending

submitter keyword: Proteomics, Tandem mass spectrometry, Mouse, Testis, Cranial irradiation

Guirong Ding
contact affiliation	School of Military Preventive Medicine, Air Force Medical University
contact email	dingzhao@fmmu.edu.cn
lab head
Ling Guo
contact affiliation	School of Military Preventive Medicine, Air Force Medical University
contact email	guolingclover@163.com
dataset submitter

iProX dataset URI