PXD037406

PXD037406 is an original dataset announced via ProteomeXchange.

Title	An oncogene addiction phosphorylation signature and its derived scores inform tumor responsiveness to targeted therapies
Description	Oncogene addiction provides important therapeutic opportunities for precision oncology treatment strategies. To date the cellular circuitries associated with driving oncoproteins, which eventually establish the phenotypic manifestation of oncogene addiction remain largely unexplored. We employed a targeted mass spectrometry approach to systematically explore alterations in 116 phosphosites related to oncogene signaling and its intersection with the DDR following inhibition of the addicting oncogene alone or in combination with irradiation in MET-, EGFR-, ALK- or BRAF (V600)-positive cancer models and ex vivo non-small cell lung cancer patient organotypic cultures. We identified an ‘oncogene addiction phosphorylation signature’ (OAPS) consisting of 8 protein phosphorylations (ACLY S455, IF4B S422, IF4G1 S1231, LIMA1 S490, MYCN S62, NCBP1 S22, P3C2A S259 and TERF2 S365) that are significantly suppressed upon targeted oncogene inhibition solely in addicted cell line models and patient tissues. We show that the OAPS is present in patient tissues and the OAPS-derived score strongly correlates with the ex vivo responses to targeted treatments.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:42:43.947.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michaela Medova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q TRAP

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-13 15:35:31	ID requested
1	2023-03-11 04:01:16	announced
⏵ 2	2023-11-14 08:42:45	announced	2023-11-14: Updated project metadata.

Orlando E, Medo M, Bensimon A, Quintin A, Riedo R, Roth SM, Riether C, Marti TM, Aebersold DM, Medov, á M, Aebersold R, Zimmer Y, An oncogene addiction phosphorylation signature and its derived scores inform tumor responsiveness to targeted therapies. Cell Mol Life Sci, 80(1):6(2022) [pubmed]

submitter keyword: oncogene addiction, phosphosignature, human, selected reaction monitoring

Yitzhak Zimmer
contact affiliation	University of Bern, Inselspital
contact email	yitzhak.zimmer@insel.ch
lab head
Michaela Medova
contact affiliation	University of Bern, Inselspital Bern
contact email	michaela.medova@dbmr.unibe.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037406

PRIDE project URI

• PRIDE
  1. PXD037406
     1. Label: PRIDE project
     2. Name: An oncogene addiction phosphorylation signature and its derived scores inform tumor responsiveness to targeted therapies