PXD037385

PXD037385 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Molecular mechanisms of endothelial osteoinductive/osteosupressive properties
Description	In vivo, osteogenesis is tightly connected with capillaries and endothelium assumed to be one of the main regulators of osteogenic differentiation of underling mesenchymal cell. In our experiments in vitro, we cultured human osteoblasts and human umbilical vein cord endothelial cells (HUVEC) in osteogenic conditions in direct joint culture and in separated joint culture where cells were separated with membrane permeable for proteins, but not for the cells. We revealed that in direct co-cultivation endothelial cells enhance osteoblast differentiation, but in separated co-cultures endothelium completely inhibit osteogenic differentiation. To understand molecular mechanisms of these osteoinductive/osteosupressive properties we performed shotgun proteomics analysis of control cultures and cells from joint cultures. To separate cells from direct co-culture we used magnetic sorting.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:28:49.019.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arseniy Lobov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-13 14:55:21	ID requested
⏵ 1	2025-05-06 09:28:49	announced

Publication List

Perepletchikova D, Kuchur P, Basovich L, Khvorova I, Lobov A, Azarkina K, Aksenov N, Bozhkova S, Karelkin V, Malashicheva A, Endothelial-mesenchymal crosstalk drives osteogenic differentiation of human osteoblasts through Notch signaling. Cell Commun Signal, 23(1):100(2025) [pubmed]
10.1186/s12964-025-02096-0;

Perepletchikova D, Kuchur P, Basovich L, Khvorova I, Lobov A, Azarkina K, Aksenov N, Bozhkova S, Karelkin V, Malashicheva A, Endothelial-mesenchymal crosstalk drives osteogenic differentiation of human osteoblasts through Notch signaling. Cell Commun Signal, 23(1):100(2025) [pubmed]

10.1186/s12964-025-02096-0;

Keyword List

submitter keyword: osteoblasts,TimsToF Pro, osteogenic differeniation, endothelium, HUVEC

submitter keyword: osteoblasts,TimsToF Pro, osteogenic differeniation, endothelium, HUVEC

Contact List

Anna Malashicheva
contact affiliation	head of Laboratory of Regenerative Biomedicine, Institute of Cytology, Russian Academy of Sciences
contact email	amalashicheva@gmail.com
lab head
Arseniy Lobov
contact affiliation	Yifat Merbl Lab, Dept. of Systems Immunology, Weizmann Institute of Science
contact email	arseniylobov@gmail.com
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD037385

PRIDE project URI

Repository Record List

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