PXD037385 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Molecular mechanisms of endothelial osteoinductive/osteosupressive properties |
Description | In vivo, osteogenesis is tightly connected with capillaries and endothelium assumed to be one of the main regulators of osteogenic differentiation of underling mesenchymal cell. In our experiments in vitro, we cultured human osteoblasts and human umbilical vein cord endothelial cells (HUVEC) in osteogenic conditions in direct joint culture and in separated joint culture where cells were separated with membrane permeable for proteins, but not for the cells. We revealed that in direct co-cultivation endothelial cells enhance osteoblast differentiation, but in separated co-cultures endothelium completely inhibit osteogenic differentiation. To understand molecular mechanisms of these osteoinductive/osteosupressive properties we performed shotgun proteomics analysis of control cultures and cells from joint cultures. To separate cells from direct co-culture we used magnetic sorting. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-06 |
AnnouncementXML | Submission_2025-05-06_09:28:49.019.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Arseniy Lobov |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-13 14:55:21 | ID requested | |
⏵ 1 | 2025-05-06 09:28:49 | announced | |
Publication List
Perepletchikova D, Kuchur P, Basovich L, Khvorova I, Lobov A, Azarkina K, Aksenov N, Bozhkova S, Karelkin V, Malashicheva A, Endothelial-mesenchymal crosstalk drives osteogenic differentiation of human osteoblasts through Notch signaling. Cell Commun Signal, 23(1):100(2025) [pubmed] |
10.1186/s12964-025-02096-0; |
Keyword List
submitter keyword: osteoblasts,TimsToF Pro, osteogenic differeniation, endothelium, HUVEC |
Contact List
Anna Malashicheva |
contact affiliation | head of Laboratory of Regenerative Biomedicine, Institute of Cytology, Russian Academy of Sciences |
contact email | amalashicheva@gmail.com |
lab head | |
Arseniy Lobov |
contact affiliation | Yifat Merbl Lab, Dept. of Systems Immunology, Weizmann Institute of Science |
contact email | arseniylobov@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037385
- Label: PRIDE project
- Name: Molecular mechanisms of endothelial osteoinductive/osteosupressive properties