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PXD037385

PXD037385 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMolecular mechanisms of endothelial osteoinductive/osteosupressive properties
DescriptionIn vivo, osteogenesis is tightly connected with capillaries and endothelium assumed to be one of the main regulators of osteogenic differentiation of underling mesenchymal cell. In our experiments in vitro, we cultured human osteoblasts and human umbilical vein cord endothelial cells (HUVEC) in osteogenic conditions in direct joint culture and in separated joint culture where cells were separated with membrane permeable for proteins, but not for the cells. We revealed that in direct co-cultivation endothelial cells enhance osteoblast differentiation, but in separated co-cultures endothelium completely inhibit osteogenic differentiation. To understand molecular mechanisms of these osteoinductive/osteosupressive properties we performed shotgun proteomics analysis of control cultures and cells from joint cultures. To separate cells from direct co-culture we used magnetic sorting.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_09:28:49.019.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterArseniy Lobov
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-13 14:55:21ID requested
12025-05-06 09:28:49announced
Publication List
Perepletchikova D, Kuchur P, Basovich L, Khvorova I, Lobov A, Azarkina K, Aksenov N, Bozhkova S, Karelkin V, Malashicheva A, Endothelial-mesenchymal crosstalk drives osteogenic differentiation of human osteoblasts through Notch signaling. Cell Commun Signal, 23(1):100(2025) [pubmed]
10.1186/s12964-025-02096-0;
Keyword List
submitter keyword: osteoblasts,TimsToF Pro, osteogenic differeniation, endothelium, HUVEC
Contact List
Anna Malashicheva
contact affiliationhead of Laboratory of Regenerative Biomedicine, Institute of Cytology, Russian Academy of Sciences
contact emailamalashicheva@gmail.com
lab head
Arseniy Lobov
contact affiliationYifat Merbl Lab, Dept. of Systems Immunology, Weizmann Institute of Science
contact emailarseniylobov@gmail.com
dataset submitter
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Dataset FTP location
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