PXD037383

PXD037383 is an original dataset announced via ProteomeXchange.

Title	Validation of the anti-Ro60 antibody (sc-100844, Sants Cruz Biotechnology, Inc) by coimmunoprecipitation from total HEK293 cell lysates
Description	The mouse monoclonal anti-Ro60 antibody (sc-100844, Sants Cruz Biotechnology, Inc) has been validated by coimmunoprecipitation from total Flp-In T-REx 293 cell lysates. An anti-His antibody (SAB1305538, Sigma-Aldrich) was used as a negative control (see experimental details in Jeandard et al., "CoLoC-seq probes the global topology of organelle transcriptomes", manuscript in preparation). Ro60 was found to be the most strongly enriched protein (~23-fold enrichment). Low-level co-enrichment of other proteins (including mitochondria-localised ones) has also been observed. These data validate the specificity of the anti-Ro60 antibody.
HostingRepository	PRIDE
AnnounceDate	2022-10-14
AnnouncementXML	Submission_2022-10-14_05:53:34.637.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037383
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	LaurianeKuhn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-13 14:54:43	ID requested
⏵ 1	2022-10-14 05:53:35	announced

10.6019/PXD037383;

submitter keyword: Ro60, antibody, TROVE2, sc-100844, CoLoC-seq

AlexandreSmirnov
contact affiliation	UMR7156 - Molecular Genetics, Genomics, Microbiology, University of Strasbourg, CNRS, Strasbourg F-67000, France.
contact email	alexandresmirnov@unistra.fr
lab head
LaurianeKuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037383
     1. Label: PRIDE project
     2. Name: Validation of the anti-Ro60 antibody (sc-100844, Sants Cruz Biotechnology, Inc) by coimmunoprecipitation from total HEK293 cell lysates