PXD037378

PXD037378 is an original dataset announced via ProteomeXchange.

Title	Quantification of membrane protein expression in G0 and G1 cell cycle phase samples in PC3 prostate cancer cells
Description	Prostate cancer (PCa) has a significant ability to disseminate and survive in the bone marrow (BM). Once there, disseminated tumor cells (DTCs) may lie dormant for years, but often eventually reactivate proliferative pathways, thus largely contributing to bone metastasis, disease progression, and relapse. Unfortunately, quiescent PCa cells are difficult to identify and target with treatment, in part because formerly identified relevant markers are intracellular and regulated by protein stability. We sought to address this problem by identifying a G0 marker that is expressed on the cell surface and therefore amenable to antibody binding for identification and therapeutic targeting. In doing so, we utilized stably transduced fluorescent markers for CDKN1B (p27) and CDT1 protein levels, which separate viable PCa cells into G0, G1, or combined S/G2/M populations (as described in PMID 34957090). We then performed FACS to collect G1 and G0 PC3 PCa cells, isolated membrane proteins, and subsequently used GC-MS proteomics to determine differences in protein abundance between G0 and G1. In total, 3,791 proteins were identified.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:24:52.072.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037378
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Henriette Remmer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-13 14:48:36	ID requested
1	2023-03-03 07:22:05	announced
⏵ 2	2023-11-14 08:24:53	announced	2023-11-14: Updated project metadata.

10.6019/PXD037378;

Yumoto K, Rashid J, Ibrahim KG, Zielske SP, Wang Y, Omi M, Decker AM, Jung Y, Sun D, Remmer HA, Mishina Y, Buttitta LA, Taichman RS, Cackowski FC, HER2 as a potential therapeutic target on quiescent prostate cancer cells. Transl Oncol, 31():101642(2023) [pubmed]

submitter keyword: Dormancy, Disseminated Tumor Cells, Quiescent, ERBB2, HER2, G0, Cell Cycle, G1, Prostate Cancer

Henriette Remmer
contact affiliation	Henriette A. Remmer, Ph.D. Director, Proteomics & Peptide Synthesis Core University of Michigan C570C MSRB II SPC 5674 1150 W. Medical Center Drive Ann Arbor, MI 48109 https://brcf.medicine.umich.edu/cores/proteomics-peptide-synthesis/
contact email	HRemmer@umich.edu
lab head
Henriette Remmer
contact affiliation	University of Michigan
contact email	hremmer@umich.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037378
     1. Label: PRIDE project
     2. Name: Quantification of membrane protein expression in G0 and G1 cell cycle phase samples in PC3 prostate cancer cells