PXD037375

PXD037375 is an original dataset announced via ProteomeXchange.

Title	Characterization of extracellular vesicles from Bothrops jararaca snake venom
Description	Venoms are important evolutionary keys and innovation for several animal taxons., including snakes. Their use is specially related to feed, predation and defense. Snake venoms are composed mainly by free secreted proteins, about 98%, and stored in the lumen of the venom gland, where they are processed through poorly understood mechanisms. In the perspective of protein-diversity, venoms undergo evolutionary pressure which generate a rapid evolutionary response, causing a great diversity in their toxin-components. We know now that extracellular vesicles exist in snake venom, although their biological role are still unknown. We believe that understanding EV-mediated effects could change our way of seeing envenoming, especially long-term sequelae. From what we know about EVs and snake venoms, there is a lot of potential of cross-organism communication occurrence between snakes and human victims. To advance in the comprehension of venom EVs function, we used fresh B. jararaca venom as our model. Fresh venom was fractionated by sequential centrifugation, resulting in two populations of vesicles (Bj-EVs). Purified Bj-EVs were analyzed by electron microscopy, NTA and proteomics. The interaction of Bj-EVs with mammalian cells was accessed by fluorescence and electron microscopy.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:23.586.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Larissa Gonçalves Machado
SpeciesList	scientific name: Bothrops jararaca (Jararaca) (Bothrops jajaraca); NCBI TaxID: 8724;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-13 14:38:06	ID requested
1	2023-03-10 14:10:44	announced
⏵ 2	2023-11-14 08:28:24	announced	2023-11-14: Updated project metadata.

Gon, ç, alves-Machado L, Ver, ç, oza BRF, Nogueira FCS, Melani RD, Domont GB, Rodrigues SP, Rodrigues JCF, Zingali RB, Venom Are Diverse in Structure and Protein Composition and Interact with Mammalian Cells. Toxins (Basel), 14(11):(2022) [pubmed]

submitter keyword: extracellular vesicles, snake venom

Russolina Benedeta Zingali
contact affiliation	Instituto de Bioquímica Médica Leopoldo de Meis, Laboratório de Hemostase e Venenos, Universidade Federal do Rio de Janeiro (UFRJ), Brazil.
contact email	lzingali@bioqmed.ufrj.br
lab head
Larissa Gonçalves Machado
contact affiliation	Universidade Federal do Rio de Janeiro (UFRJ), Brazil
contact email	larissa.machado@bioqmed.ufrj.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037375
     1. Label: PRIDE project
     2. Name: Characterization of extracellular vesicles from Bothrops jararaca snake venom