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PXD037368

PXD037368 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComprehensive characterization of protein turnover by comparative SILAC labeling analysis in 3T3-L1
DescriptionA balance between the synthesis and degradation of proteins is referred to as protein turnover, which is crucial for cellular protein homeostasis. Proteome-wide analysis of protein turnover in adipocytes, which are well-known for storing energy and also being associated with obesity and metabolic disorders, are yet to be carried out. Thus, with this objective in mind, our study employed comparative analysis of time-dependent SILAC labeling to evaluate the protein turnover of 3T3-L1 adipocytes ranging from 0 to 144 hours. We observed that relatively faster or slower protein half-lives in several protein groups were related to the PPAR signaling pathway, energy metabolism, extracellular matrix, ubiquitin-proteasome system, RNA splicing, Golgi complex, and lysosome. It is anticipated that these protein turnover profiles will provide greater clarity on the life cycle of adipocyte proteome and shed light on how they maintain protein homeostasis.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:44:52.780.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSunkyu Choi
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-13 14:33:07ID requested
12023-04-17 17:37:00announced
22023-11-14 08:45:06announced2023-11-14: Updated project metadata.
Publication List
Choi S, Romero AR, Mashood F, Goswami N, Chouchane L, Schmidt F, Comprehensive Characterization of Protein Turnover by Comparative SILAC Labeling Analysis in 3T3-L1. J Proteome Res, 22(6):1723-1733(2023) [pubmed]
Keyword List
submitter keyword: Mass Spectrometry, Protein turnover, Adipocytes,Proteomics, SILAC
Contact List
Sunkyu Choi
contact affiliationWeill Cornell Medicine-Qatar
contact emailszc2010@qatar-med.cornell.edu
lab head
Sunkyu Choi
contact affiliationWeill Cornell Medicine-Qatar
contact emailszc2010@qatar-med.cornell.edu
dataset submitter
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Dataset FTP location
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