PXD037366
PXD037366 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | THE EFFECT OF AGE ON THE PROTEOMIC PROFILE OF EQUINE BRONCHOALVEOLAR LAVAGE FLUID |
| Description | Neonates have different cellular composition in their bronchoalveolar lavage fluid (BALF) when compared to older foals and adult horses; however, little is known about the non-cellular components of BALF. The objective of this study was to determine the proteomic composition of BALF in neonatal horses and to compare it to that of older foals and adult horses. Bronchoalveolar lavage fluid samples of seven neonates (< 1 week age), four 5 to 7-week-old foals, and six adult horses were collected. Quantitative Proteomics of the fluid was performed using tandem mass tag labeling followed by LC-MS/MS and protein relative expressions were compared between groups. A total of 704 proteins were identified with gene ontology terms and were classified. Of these, 332 proteins were related to the immune system in neonates, foals, and adult horses. The most frequent molecular functions identified were binding and catalytic activity and the most common biological processes were cellular process, metabolic process, and biological regulation. There was a significant difference in the proteome of neonates when compared to foals and to adult horses. Neonates had less relative expression (FDR < 0.01) of many immune-related proteins, including immunoglobulins, proteins involved in the complement cascade, ferritin, BPI fold-containing family B member 1, and macrophage receptor MARCO. This is the first report of neonatal foals BALF proteomics and reveals differential expression of proteins when compared to BALF from adult horses. The lower relative expression of immune-related proteins in neonates could contribute to their susceptibility to pulmonary infections |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-11-01 |
| AnnouncementXML | Submission_2023-11-01_11:10:49.961.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Brett Phinney |
| SpeciesList | scientific name: Equus caballus; common name: horse; NCBI TaxID: 9796; |
| ModificationList | TMT6plex |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-10-13 13:57:47 | ID requested | |
| ⏵ 1 | 2023-11-01 11:10:50 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: BRONCHOALVEOLAR LAVAGE FLUID |
Contact List
| Macarena G. Sanz | |
|---|---|
| contact affiliation | Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University |
| contact email | macarena@wsu.edu |
| lab head | |
| Brett Phinney | |
| contact affiliation | UC Davis |
| contact email | brettsp@ucdavis.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090531/ |
to receive all new ProteomeXchange dataset release announcements!
