PXD037364

PXD037364 is an original dataset announced via ProteomeXchange.

Title	Breaking the mucin barrier: a new affinity chromatography-mass spectrometry approach to unveil potential cell markers and pathways altered in Pseudomyxoma peritonei
Description	Pseudomyxoma peritonei (PMP) is a rare, malignant tumour with unknown underlying molecular mechanisms characterised by a progressive accumulation of mucin and secreting tumour cells within the abdomen and pelvis. Cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC) is the only therapeutic option currently available for its treatment and recurrence with a fatal outcome is common despite its use. The lack of information available about the molecular mechanisms underlying this condition is mainly due to the physicochemical characteristics of mucin, whose main purpose is to protect epithelial cells, which makes it inaccessible to a proteomic analysis. In this paper we describe the first protocol available to break this barrier and offer a chance to isolate proteins from soft and hard mucinous tumour tissues. This approach is based on the depletion of glycoproteins, immunoglobins, and albumins by affinity liquid chromatography, which makes the mucin available for label free quantification by mass spectrometry. As in the case of non mucinous tissues, our protocol yields a mix of total proteins and has allowed us to establish the first protein profile in mucinous tumours. In addition, our protein-protein interaction networks and pathway enrichment analyses revealed MUC13 and TFF1 as potential therapeutic targets in this disease.
HostingRepository	PRIDE
AnnounceDate	2024-06-22
AnnouncementXML	Submission_2024-06-22_09:05:16.532.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Trypanosoma cruzi cruzi; NCBI TaxID: 85057; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-13 06:54:31	ID requested
⏵ 1	2024-06-22 09:05:17	announced

10.1186/s12575-024-00239-0;

Romero-Ruiz A, Granados-Rodr, í, guez M, Bura FI, Valenzuela-Molina F, Rufi, á, n-And, ú, jar B, Mart, í, nez-L, ó, pez A, Rodr, í, guez-Ortiz L, Ortega-Salas R, Torres-Mart, í, nez M, Moreno-Serrano A, Casta, ñ, o J, Mich, á, n C, Alhama J, V, á, zquez-Borrego MC, Arjona-S, á, nchez Á, Breaking the Mucin Barrier: A New Affinity Chromatography-Mass Spectrometry Approach to Unveil Potential Cell Markers and Pathways Altered in Pseudomyxoma Peritonei. Biol Proced Online, 26(1):13(2024) [pubmed]

submitter keyword: SWATH, Differential expresion,Pseudomyxoma peritonei, Mucin

Antonio Romero-Ruiz
contact affiliation	GE09 research in peritoneal and retroperitoneal oncological surgery, Maimonides Biomedical Research Institute of Córdoba (IMIBIC, Instituto Maimónides de Investigación Biomédica de Córdoba), Spain.
contact email	antonioromeroruiz@gmail.com
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD037364

PRIDE project URI

• PRIDE
  1. PXD037364
     1. Label: PRIDE project
     2. Name: Breaking the mucin barrier: a new affinity chromatography-mass spectrometry approach to unveil potential cell markers and pathways altered in Pseudomyxoma peritonei