PXD037342

PXD037342 is an original dataset announced via ProteomeXchange.

Title	P bodies coat germ granules to promote transgenerational gene silencing in C. elegans
Description	Formation of biomolecular condensates have emerged as a critical mechanism for compartmentation in living cells. Despite interactions between distinct condensates have been reported, the biological relevance of such interactions remains elusive. In germ cells, small RNA silencing factors are enriched in germ granules, which distinct factors are organized into sub-compartments with specific functions linked to genome surveillance or transgenerational gene silencing. Here we showed that perinuclear germ granules are coated by P body, another condensate known for housing untranslated mRNAs and mRNA degradation factors. Disruption of P body factors, including CGH-1/DDX6 and CAR-1/LSM14, lead to dispersal of small RNA factors from perinuclear germ granules and disorganization of sub-compartments within germ granules. We further showed that CAR-1 promote the interactions between CGH-1 with germ granule factors and such interactions are critical for CGH-1’s ability to promote piRNA-mediated gene silencing. Importantly, we observed that cgh-1 mutants are competent in triggering gene silencing but exhibit defects in maintaining gene silencing effects in the subsequent generations. Small RNA sequencing further showed that cgh-1 mutants exhibit defects in amplifying secondary small RNAs, a known carrier of gene silencing memory. Together, our results uncover the function of P body factors in small RNA-mediated transgenerational gene silencing and highlight how the formation and function of one condensate can be regulated by an adjacent, interacting condensate in cells.
HostingRepository	PRIDE
AnnounceDate	2023-06-16
AnnouncementXML	Submission_2023-06-15_19:16:29.009.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DongleiZhang
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-12 14:00:41	ID requested
⏵ 1	2023-06-15 19:16:29	announced

Dataset with its publication pending

submitter keyword: CGH-1,P body, germ granule, RNAi inheritance

DongleiZhang
contact affiliation	Huazhong University of Science and Technology
contact email	zhang_donglei@hust.edu.cn
lab head
DongleiZhang
contact affiliation	Huazhong University of Science and Technology
contact email	zhang_donglei@hust.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037342
     1. Label: PRIDE project
     2. Name: P bodies coat germ granules to promote transgenerational gene silencing in C. elegans