PXD037340

PXD037340 is an original dataset announced via ProteomeXchange.

Title	Deep plasma proteome profiling by enrichment of extracellular vesicles
Description	This work show that enrichment of extracellular vesicles by ultracentrifugation increases the plasma proteome depth by an order of magnitude. Following the enrichment protocol, more than two thousand proteins can routinely be quantified reproducibly by label-free quantification and data independent acquisition (DIA) in single-shot LC-MSMS runs of less than one hour. The project also present an optimized workflow for plasma proteomics that enables high-throughput with very short chromatographic gradients analyzing hundred samples per day with deep proteome coverage, especially when including study-specific spectral libraries generated by repeated injection and gas-phase fractionation of pooled samples
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:35.545.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ole Østergaard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-12 13:57:46	ID requested
1	2022-12-03 20:47:55	announced
⏵ 2	2023-11-14 08:58:36	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mass Spectrometry,Plasma Proteomics, Extracellular Vesicles, Ultracentrifugation, Data Independent Acquisition, Biomarkers

Jesper Velgaard Olsen
contact affiliation	Novo Nordisk Foundation, Center for Protein Research Faculty of Health and Medical Sciences University of Copenhagen Blegdamsvej 3B, DK-2200 Copenhagen N Denmark
contact email	jesper.olsen@cpr.ku.dk
lab head
Ole Østergaard
contact affiliation	Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen N, Denmark
contact email	ole.ostergaard@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD037340

PRIDE project URI

• PRIDE
  1. PXD037340
     1. Label: PRIDE project
     2. Name: Deep plasma proteome profiling by enrichment of extracellular vesicles