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PXD037329

PXD037329 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleH2O2 induced oxidative stress may trigger cellular senescence in retinal photoreceptor cells through modulation of ECM-receptor interaction, oxidative phosphorylation and spliceosome pathways
DescriptionPhotoreceptor cells are highly susceptible to oxidative stress induced damage due to their high metabolic rate. Oxidative stress plays a key role in driving pathological events in several different ocular diseases which lead to retinal degeneration and ultimately blindness. A growing number of studies have been performed to understand downstream events caused by ROS induced oxidative stress in photoreceptor cells; however, the underlying mechanisms of ROS toxicity are not fully understood. Therefore, to shed light on the ROS induced downstream pathological events, we employed a tandem mass tag (TMT) labeling-based quantitative mass-spectrometric approach to determine proteome changes in 661W photoreceptor cells following oxidative stress induction via application of different concentrations of H2O2 at different time points. Overall, 5920 proteins were identified and quantified and 450 differentially expressed proteins (DEPs) were identified, which were altered in a dose and time dependent manner in all treatment groups compared to the control group. These proteins were involved in several biological pathways including spliceosome and ribosome response, activated glutathione metabolism, decreased ECM-receptor interaction, oxidative phosphorylation, abnormally regulated lysosome, apoptosis, and ribosome biogenesis. Our results highlighted ECM-receptor interaction, oxidative phosphorylation and spliceosome pathways as the major targets of oxidative stress that might mediate vascular dysfunction and cellular senescence.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:47:40.149.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLiting Deng
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-12 08:32:24ID requested
12023-05-10 14:54:22announced
22023-11-14 08:47:50announced2023-11-14: Updated project metadata.
Publication List
Deng L, Gupta V, Abyadeh M, Chitranshi N, Pushpitha K, Wu Y, Gupta V, You Y, Paulo JA, Graham SL, Mirzaei M, Haynes PA, Oxidative Stress Induced Dysfunction of Protein Synthesis in 661W Mice Photoreceptor Cells. Proteomes, 11(2):(2023) [pubmed]
Keyword List
submitter keyword: Proteomics
Photoreceptor
Oxidative stress
Retina
ECM receptor interaction signaling pathway
Cellular senescence.
Contact List
Paul A. Haynes
contact affiliationSchool of Natural Sciences,Faculty of Science and Engineering, Macquarie University
contact emailpaul.haynes@mq.edu.au
lab head
Liting Deng
contact affiliationMacquarie University
contact emailliting.deng@hdr.mq.edu.au
dataset submitter
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Dataset FTP location
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