PXD037320

PXD037320 is an original dataset announced via ProteomeXchange.

Title	Comparative analysis of proteomes and secretomes of human mesenchymal stem cells isolated from six sources during osteogenic differentiation
Description	Ability to perform osteogenic differentiation is one of the minimal criteria of mesenchymal stem cells (MSCs). Still, it is generally unknown whether osteogenic differentiation is universal cell fate or various phenotypically similar cell states. Besides this, MSCs and their secretomes are actively using for cell/cell-free therapy development, but systemic inter-source variation in MSCs secretomes, proteomes and differentiation mechanisms are still poorly understood. Therefore, here we compared proteomic and secretomic profiles of human mesenchymal cells from six sources: osteoblasts (bone), WJ-MSCs (Warton’s jelly), AD-MSCs (adipose), PDLSCs (tooth: Periodontal Ligament Stem Cells), DPSCs (tooth: Dental Pulp Stem Cells) and GFs (tooth: Gingival Fibroblasts). For experiments we used cells in early passages (3-5) isolated from 3-6 individuals. All cells were compared in standard cultivation and in the 10th day after induction of osteogenic differentiation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:13:35.012.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arseniy Lobov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-12 08:31:33	ID requested
1	2023-11-05 04:00:04	announced
⏵ 2	2023-11-14 09:13:35	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: MSC,TimsToF Pro, osteogenic differeniation, secretome

Anna Malashicheva
contact affiliation	head of Laboratory of Regenerative Biomedicine, Institute of Cytology, Russian Academy of Sciences
contact email	amalashicheva@gmail.com
lab head
Arseniy Lobov
contact affiliation	Emmanuel Levy Lab, Dept. of Chemical and Structural Biology, Weizmann Institute of Science
contact email	arseniylobov@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037320
     1. Label: PRIDE project
     2. Name: Comparative analysis of proteomes and secretomes of human mesenchymal stem cells isolated from six sources during osteogenic differentiation