PXD037318

PXD037318 is an original dataset announced via ProteomeXchange.

Title	The missed histone H1 is involved in cell division and ribosome biosynthesis in Toxoplasma gondii
Description	Chromatin dynamics can regulate all DNA-dependent processes. Access to DNA within chromatin is orchestrated mainly by histones and their posttranslational modifications (PTM). Like other eukaryotes, the apicomplexan parasite Toxoplasma gondii encodes for four canonical histones and five histone variants. By contrast, the linker histone (H1) has never been identified in apicomplexan parasites. In other eukaryotes, histone H1 compacts the chromatin by linking the nucleosome and increasing the DNA compaction. H1 is a multifunctional protein and can be involved in different steps of DNA metabolism or associated with protein complexes related to distinct biological processes. We have identified a novel protein in T. gondii that, although lacking the globular domain of mammalian H1, is remarkably like the H1-like proteins of bacteria and trypanosomatids. Our results demonstrate that TgH1-like is a nuclear protein associated with chromatin and other histones. Curiously, TgH1-like is also in the nucleolus and associated with ribosomal proteins, indicating a versatile function in this parasite. Although knockout of tgh1-like does not affect the cell cycle, it causes endopolygeny and asynchronous division. Interestingly, mutating post-translationally modified amino acids results in defects in cell division like ∆tgh1-like, showing that these sites are important for protein function. Furthermore, in the bradyzoite stage, this protein is expressed only in dividing parasites, reinforcing its importance in cell division. Indeed, the absence of TgH1-like decreases compaction of peripheral chromatin, confirming its role in the chromatin modulation in T. gondii.
HostingRepository	PRIDE
AnnounceDate	2022-11-30
AnnouncementXML	Submission_2022-11-30_07:59:13.225.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JuliaCunha
SpeciesList	scientific name: Toxoplasma gondii VEG; NCBI TaxID: 432359;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-12 08:31:20	ID requested
⏵ 1	2022-11-30 07:59:13	announced

Dataset with its publication pending

submitter keyword: Toxoplasma gondii, cell division, histone H1, chromatin, posttranslational modification

SheilaNardelli
contact affiliation	Laboratório de Pesquisa em Apicomplexa - Instituto Carlos Chagas – FIOCRUZ/PR.
contact email	shenardelli@gmail.com
lab head
JuliaCunha
contact affiliation	LCC
contact email	julia.cunha@butantan.gov.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD037318

PRIDE project URI

• PRIDE
  1. PXD037318
     1. Label: PRIDE project
     2. Name: The missed histone H1 is involved in cell division and ribosome biosynthesis in Toxoplasma gondii