PXD037308

PXD037308 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based proteomic study discovered LAMP2 to be a novel biomarker related to HIV-1 latency
Description	Purpose: This study aimed to identify potential biomarkers related to HIV latency on the cell surface. Experimental design: A subcellular proteomic study was performed, using an HIV-1 latency cell line (U1 (HIV-1-integrated U937 cell line)) and control (U937). Plasma membrane proteins were enriched, digested, labeled using an iTRAQ kit, and identified by LC-MS. Differentially expressed proteins were analyzed using bioinformatics technology, followed by western blot validation in cell lines and resting CD4+ T cells from patients. Results: Totally, 110 differentially expressed proteins were identified in U1 compared with U937. Bioinformatics analysis suggested that the immune response and phagosomes were associated with the altered proteins, and HIV proteins such as Vpr were involved in the regulation of these differential proteins. Lysosome-associated membrane glycoprotein 2 (LAMP2), complement decay-accelerating factor, integrin alpha-6, and leukocyte surface antigen CD47 were shown to be downregulated in HIV-1 latent cell lines. Of these, LAMP2 was also shown to be downregulated in enriched resting CD4+ T cells from HIV-infected patients compared with those from healthy volunteers. Conclusions and clinical relevance: Our results indicate that HIV might decrease immunity proteins such as LAMP2 and CD47 to avoid immune recognition. LAMP2 may be a potential biomarker for HIV-1 latency.
HostingRepository	iProX
AnnounceDate	2022-10-12
AnnouncementXML	Submission_2022-10-12_01:19:45.694.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lijun zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-12 01:17:49	ID requested
⏵ 1	2022-10-12 01:19:46	announced

Dataset with its publication pending

submitter keyword: proteomics, plasma membrane, HIV latency, iTRAQ, LAMP2

Lijun zhang
contact affiliation	Shanghai Public Health Clinical Center
contact email	zhanglijun5028@dingtalk.com
lab head
Lijun zhang
contact affiliation	Shanghai Public Health Clinical Center
contact email	zhanglijun5028@dingtalk.com
dataset submitter

iProX dataset URI