PXD037289

PXD037289 is an original dataset announced via ProteomeXchange.

Title	Proteins associated wth Trypanosoma brucei HNRNPF/H
Description	We aimed to find proteins associated with HNRNPH in bloodstream-form _Trypanosoma brucei_. We integrated a sequence encoding a cleavable TAP (Tandem Affinity Purification) tag into the genome upstream of, and in frame with, one _HNRNPH_ gene. The other allele was deleted. TAP-HNRNPH was purifed from triplicate lysates as shown under "Sample processing protocol". Sample processing protocol (30-5000chracters) The TAP-HNRNPH was bound to IgG beads, then released with Tobacco Etch Virus (TEV) protease. TAP-CFP served as the negative control. The His-tagged TEV protease was then removed using Ni-NTA-magnetic beads. Eluted proteins were separated on a 1.5 mm NuPAGE™ Novex™ 4-12 % Bis-Tris protein gel. For details see doi: 10.1371/journal.pone.0258903
HostingRepository	PRIDE
AnnounceDate	2022-10-15
AnnouncementXML	Submission_2022-10-14_18:56:33.097.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ThomasRuppert
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-11 03:57:05	ID requested
⏵ 1	2022-10-14 18:56:33	announced

Dataset with its publication pending

submitter keyword: Trypanosoma brucei

mRNA

RNA-binding protein

trans splicing

mRNA decay

ThomasRuppert
contact affiliation	University Heidelberg, ZMBH, Mass spectromerty and proteomics, Im Neuenheimer Feld 282 69120 Heidelberg, Germany
contact email	t.ruppert@zmbh.uni-heidelberg.de
lab head
ThomasRuppert
contact affiliation	ZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg
contact email	t.ruppert@zmbh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD037289

PRIDE project URI

• PRIDE
  1. PXD037289
     1. Label: PRIDE project
     2. Name: Proteins associated wth Trypanosoma brucei HNRNPF/H