PXD037286

PXD037286 is an original dataset announced via ProteomeXchange.

Title	Surveying the chloroplast protein kinase preferences for cation cofactors in Arabidopsis thaliana
Description	Survival of plants depends on their ability to adapt to ever-changing environmental conditions. Chloroplasts sense different stimuli and respond to ion fluxes in the cytosol triggering systemic responses under stress conditions. Indeed, changes in calcium and magnesium ion concentration in the chloroplast stroma impact the immune response against pathogens and adapt the photosynthetic machinery under fluctuating light conditions. Here we investigated the link between di-valent cations, calcium, magnesium and manganese and the protein kinase activity in Arabidopsis chloroplasts. Our results show that overall, manganese ions are the strongest activator of kinase activity in chloroplasts followed by magnesium ions, whereas calcium ions have seemingly no effect. Additionally, when comparing the phosphorylation pattern from Arabidopsis wild type and a cmt1 mutant, which is defective in manganese import into chloroplasts, the phosphorylation of specific protein bands is strongly reduced in the mutant chloroplasts supporting the notion that chloroplasts contain manganese-dependent protein kinases. These findings provide insights for the future characterization of chloroplast protein kinases activity regarding in-vitro assays and potential target proteins.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:38:39.360.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Serena Schwenkert
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-11 03:55:52	ID requested
1	2023-03-10 23:36:47	announced
⏵ 2	2023-11-14 08:38:40	announced	2023-11-14: Updated project metadata.

Espinoza-Corral R, Schwenkert S, Schneider A, Characterization of the preferred cation cofactors of chloroplast protein kinases in Arabidopsis thaliana. FEBS Open Bio, 13(3):511-518(2023) [pubmed]

submitter keyword: Arabidopsis,chloroplast, maganese

Serena Schwenkert
contact affiliation	Biozentrum der LMU München Pflanzenwissenschaften Großhaderner Str. 2-4 82152 Planegg-Martinsried phone +49 +89 2180 74760
contact email	serena.schwenkert@lmu.de
lab head
Serena Schwenkert
contact affiliation	LMU Munich
contact email	serena.schwenkert@lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037286

PRIDE project URI

• PRIDE
  1. PXD037286
     1. Label: PRIDE project
     2. Name: Surveying the chloroplast protein kinase preferences for cation cofactors in Arabidopsis thaliana