Braun’s lipoprotein (Lpp) plays a major role in stabilizing the integrity of the cell envelope in E. coli, as it provides a covalent crosslink between the outer membrane and the peptidoglycan layer. Here, using atomic force microscopy, we visualized Lpp directly on peptidoglycan sacculi. Lpp is homogeneously distributed over the outer surface of the sacculus at a high density.