PXD037251

PXD037251 is an original dataset announced via ProteomeXchange.

Title	Fc glycosylation status of Vi vaccine-induced antibodies following polysaccharide or conjugate Salmonella Typhi vaccine in UK adults and Nepalese children
Description	Conjugate vaccine against typhoid fever has been shown to be safe and effective in field trials. The mechanism through which the vaccine protects remains elusive. Recent data have implicated antibody glycosylation, and specifically afucosylated antibodies, as an important factor in vaccine-induced effector function for a range of viral infections. Here, we studied IgG glycosylation after conjugate vaccine in a UK cohort, who were then challenged with virulent S.typhi, and among Nepalese children living in a typhoid endemic region. We compared vaccine-induced responses to another licensed typhoid polysaccharide vaccine and correlated these measures with antibody-dependent function to understand if a vaccine against a bacterial infection elicited similar glycosylation/function associations as has been seen for viral infections. Robust antigen-specific IgG Fc galactosylation and sialylation modifications were induced by both polysaccharide and tetanus toxoid-conjugated Salmonella Typhi vaccines in UK adults. These modifications were not able to differentiate controlled human infection model (CHIM) participants who became ill after ingestion of virulent S.typhi from those who remained well after infection. However, among those who did become ill, disease severity was associated with a distinct glycosylation profile. Interestingly, both vaccines induced vaccine-specific IgG1 antibodies that were more fucosylated than total circulating IgG1 and these were not associated with a particular functional profile. While bulk IgG glycosylation was different between Nepalese children and UK adults, vaccination with the Vi-tetanus toxoid-conjugate vaccine resulted in similar Vi-specific IgG glycosylation profiles 28 days after vaccination in both cohorts.
HostingRepository	PRIDE
AnnounceDate	2024-05-30
AnnouncementXML	Submission_2024-05-30_01:57:33.042.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Noortje de Haan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-10 07:43:29	ID requested
⏵ 1	2024-05-30 01:57:33	announced

10.3389/FRANS.2022.1005558;

submitter keyword: IgG, glycan, Thyphoid fever, vaccine

Noortje de Haan
contact affiliation	Center for Proteomics and Metabolomics, Leiden Universtity Medical Center, The Netherlands
contact email	n.de_haan@lumc.nl
lab head
Noortje de Haan
contact affiliation	Copenhagen center for Glycomics, University of Copenhagen
contact email	ndehaan@sund.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037251
     1. Label: PRIDE project
     2. Name: Fc glycosylation status of Vi vaccine-induced antibodies following polysaccharide or conjugate Salmonella Typhi vaccine in UK adults and Nepalese children