PXD037245

PXD037245 is an original dataset announced via ProteomeXchange.

Title	Peptidomic analysis of endogenous and bacterial protease activity in human plasma
Description	The clinical assessment of wound infection and related complications is challenging and the development of objective methods to measure wound status and predict healing outcomes is therefore essential. As endogenous and bacterial protease activities play important roles in healing and infection, analysis of changes in the wound peptidome by individual enzymes could provide insight into proteolytic events occurring in wounds, and may aid in the discovery of biomarkers. We mimicked wound fluid formation and wound infection by digesting plasma ex vivo with endogenous (human neutrophil elastase and Cathepsin G) and bacterial proteases (Pseudomonas aeruginosa LasB and Staphyloccocus aureus V8). Using a peptidomics approach, we characterized the low-molecular-weight peptidome of these samples and identified over 100 protein targets for each enzyme, and found enzyme-specific peptides and digestion patterns.
HostingRepository	PRIDE
AnnounceDate	2024-03-07
AnnouncementXML	Submission_2024-03-07_02:08:13.269.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariena van der Plas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-10 07:33:21	ID requested
⏵ 1	2024-03-07 02:08:14	announced

10.1016/j.isci.2024.109005;

Cai J, Nielsen MW, Kalogeropoulos K, Auf dem Keller U, van der Plas MJA, Peptidomic analysis of endogenous and bacterial protease activity in human plasma and wound fluids. iScience, 27(2):109005(2024) [pubmed]

submitter keyword: Human, LC-MSMS, plasma, proteases, peptidome

Mariena J.A.
contact affiliation	LEO Foundation Center for Cutaneous Drug Delivery Department of Pharmacy University of Copenhagen Denmark
contact email	mariena.van_der_plas@sund.ku.dk
lab head
Mariena van der Plas
contact affiliation	University of Copenhagen Department of Pharmacy
contact email	mariena.van_der_plas@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD037245

PRIDE project URI

• PRIDE
  1. PXD037245
     1. Label: PRIDE project
     2. Name: Peptidomic analysis of endogenous and bacterial protease activity in human plasma