PXD037244

PXD037244 is an original dataset announced via ProteomeXchange.

Title	PDI-catalyzed oxidative protein folding mediates senescence by induction of ER-derived oxidative stress
Description	Protein homeostasis is linked with aging and aging associated diseases. Oxidative protein folding occurs in the endoplasmic reticulum (ER) and produces H2O2 as a byproduct. The role of oxidative protein folding in human stem cells aging remains unknown. Here, we succeed to knockout protein disulfide isomerase (PDI), a key oxidoreductase for catalyzing oxidative protein folding, in human embryonic stem cells (hESCs) and human mesenchymal stem cells (hMSCs). Deletion of PDI does not affect the self-renewal of hESCs but significantly delays hMSCs senescence. Mechanistically, knockout of PDI slows down the rate of oxidative protein folding and decreases the leakage of ER-derived H2O2 into the nucleus, therefore alleviating oxidative stress and the secretory-associated senescence phenotype (SASP). Among the SASP-related genes, SERPINE1 is identified as a key driver for cell senescence. These findings establish a novel link between oxidative protein folding and aging. The previously unrecognized function of PDI in regulating cell senescence provides a potential target for aging and aging-related disease intervention.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:07.303.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ji zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-10 07:32:54	ID requested
1	2023-06-12 13:16:50	announced
⏵ 2	2023-11-14 09:01:07	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: human mesenchymal stem cells (hMSCs)

hydrogen peroxide (H2O2)

oxidative 29 protein folding

protein disulfide isomerase (PDI)

senescence

Qianzhao Ji
contact affiliation	CAS（Chinese Academy of Sciences）
contact email	jiqianzhaonotold@outlook.com
lab head
ji zhao
contact affiliation	17812007228
contact email	jiqianzhaonotold@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD037244

PRIDE project URI

• PRIDE
  1. PXD037244
     1. Label: PRIDE project
     2. Name: PDI-catalyzed oxidative protein folding mediates senescence by induction of ER-derived oxidative stress