PXD037236

PXD037236 is an original dataset announced via ProteomeXchange.

Title	Lung extracellular matrix modulates KRT5+ basal cell activity in pulmonary fibrosis
Description	Aberrant expansion of KRT5+ basal cells in the distal lung accompanies progressive alveolar epithelial cell loss and tissue remodelling during fibrogenesis in idiopathic pulmonary fibrosis (IPF). The mechanisms determining activity of KRT5+ cells in IPF have not been delineated. Here, we show an association between KRT5+ cells in human fibrotic lung and regional differences in collagen topography. In vitro, KRT5+ cell migratory characteristics and expression of remodelling genes are modulated by extracellular matrix (ECM) composition and organisation. Mass spectrometry-based proteomics revealed compositional differences in the matrisome secreted by primary human lung fibroblasts (HLF) from IPF patients compared to controls. Over-expression of ECM glycoprotein, Secreted Protein Acidic and Cysteine Rich (SPARC) in the IPF HLF matrisome restricts KRT5+ cell migration in vitro. Together, our findings demonstrate that changes to the ECM in IPF directly influence KRT5+ cell behaviour and function contributing to remodelling events in the fibrotic niche.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:06.329.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maryline Fresquet
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-10 07:19:54	ID requested
1	2023-09-28 01:11:15	announced
⏵ 2	2023-11-14 08:49:07	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, ECM, lung, fibrosis

Clare Lloyd
contact affiliation	National Heart and Lung Institute, Imperial College London, London, SW7 2AZ, UK
contact email	c.lloyd@imperial.ac.uk
lab head
Maryline Fresquet
contact affiliation	University of Manchester
contact email	maryline.fresquet@manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037236
     1. Label: PRIDE project
     2. Name: Lung extracellular matrix modulates KRT5+ basal cell activity in pulmonary fibrosis