PXD037223

PXD037223 is an original dataset announced via ProteomeXchange.

Title	Mutation in a chlorophyll binding motif of Brassica ferrochelatase enhances both heme and chlorophyll biosynthesis
Description	The heme branch of tetrapyrrole biosynthesis contributes to the regulation of chlorophyll levels. However, the molecular mechanism underlying the balance between chlorophyll and heme synthesis remains elusive. Here, we identified a dark green leaf mutant dg, which contains high levels of chlorophyll b, from an ethyl methanesulfonate (EMS) -induced mutant library of Chinese cabbage. The dg phenotype is attributed to a nonsynonymous homozygous mutation in ferrochelatase 2 (BrFC2), leading to an arginine (R) to lysine (K) amino acid substitution in the conserved chlorophyll a/b-binding motif (CAB) of BrFC2. Overexpression of mutated BrFC2 (dBrFC2) in Chinese cabbage results in a pronounced phenotype associated with the dark green leaves, in contrast to transgenic lines overexpressing wild-type BrFC2 with a low-chlorophyll phenotype. Increased dBrFC2 homodimer formation stimulates heme production in dg compared with wildtype BrFC2. Moreover, we found that dBrFC2 interacts with protochlorophyllide oxidoreductase B1 and B2 (BrPORB1 and BrPORB2) to enhance their binding ability to substrate protochlorophyllide (Pchlide), thereby promoting BrPORBs-catalyzed production of chlorophyllide (Chlide) that can be directly converted into chlorophyll. Our results reveal that dBrFC2 is a strong gain-of-function mutation contributing to balancing heme and chlorophyll synthesis via a novel regulatory mechanism in which dBrFC2 promotes BrPORB enzymatic reaction to enhance chlorophyll synthesis.
HostingRepository	iProX
AnnounceDate	2022-10-09
AnnouncementXML	Submission_2022-10-09_21:10:17.362.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mengyang Liu
SpeciesList	scientific name: Brassica rapa subsp. pekinensis; NCBI TaxID: 51351;
ModificationList	iTRAQ8plex reporter+balance reagent acylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-09 21:10:06	ID requested
⏵ 1	2022-10-09 21:10:17	announced

Dataset with its publication pending

submitter keyword: Brassica，Chinese cabbage， chloroplast， ferrochelatase

Jianjun Zhao
contact affiliation	Hebei Agricultural University
contact email	jjz1971@aliyun.com
lab head
Mengyang Liu
contact affiliation	Hebei Agricultural University
contact email	liumengyang_7@163.com
dataset submitter

iProX dataset URI