PXD037210

PXD037210 is an original dataset announced via ProteomeXchange.

Title	Identification of ALK3-associated proteins
Description	The epitope-tagging strategy to isolate ALK3-interacting protein complexes was performed. In brief, 293T cells were transfected with pcDNA3.1-FLAG-ALK3 for 48 hours. The cell pellets were resuspended in IP lysis buffer, and centrifuged at 12,000 rpm for 30 minutes at 4 °C. The cell lysates were filtered with 0.45 μm syringe filters, and then the supernatants were incubated with anti-FLAG antibody or normal IgG (negative control) overnight at 4 °C, and then incubated with Protein A/G agarose beads (sc-2003, Santa Cruz) for 6 hours at 4 °C. After that, the beads were washed with IP lysis buffer for 3 times and collected, and glycine-HCl solution (pH=3.5) was used to elute the bound polypeptides. The final eluates were subjected to an in-solution digestion and further mass spectrometry peptide sequencing.
HostingRepository	iProX
AnnounceDate	2022-10-08
AnnouncementXML	Submission_2022-10-07_22:48:26.486.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zeyu Cai
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	MaxQuant

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-07 22:47:59	ID requested
⏵ 1	2022-10-07 22:48:26	announced

Dataset with its publication pending

submitter keyword: ALK3

Zeyu Cai
contact affiliation	Peking University
contact email	caizy@bjmu.edu.cn
lab head
Zeyu Cai
contact affiliation	Peking University
contact email	caizy@bjmu.edu.cn
dataset submitter

iProX dataset URI