PXD037184

PXD037184 is an original dataset announced via ProteomeXchange.

Title	An RNA granule for translation quality control in Saccharomyces cerevisiae
Description	Cytoplasmic RNA granules compartmentalize phases of the translation cycle in eukaryotes. We previously reported the localization of oxidized RNA to cytoplasmic foci called oxidized RNA bodies (ORBs) in human cells. We show here that ORBs are RNA granules in Saccharomyces cerevisiae. Several lines of evidence support a role of ORBs in the compartmentalization of no-go decay and ribosome quality control, the translation quality control pathways that recognize and clear aberrant mRNAs, including those with oxidized bases. Translation is required by these pathways and ORBs. Translation quality control factors localize to ORBs. A substrate of translation quality control, a stalled mRNA-ribosome-nascent chain complex, localizes to ORBS. Translation quality control mutants have altered ORB numbers, sizes, or both. In addition, we identify 68 ORB proteins, by immunofluorescence staining directed by proteomics, which further support their role in translation quality control and reveal candidate new factors for these pathways.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:04.545.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037184
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jamieson Dhaliwal
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-05 00:50:02	ID requested
1	2022-11-23 07:44:38	announced
⏵ 2	2023-11-14 08:59:05	announced	2023-11-14: Updated project metadata.

10.6019/PXD037184;

submitter keyword: translation.,RNA granules, oxidized RNA, ribosome-associated quality control, no-go decay

William Zerges
contact affiliation	Department of Biology, Concordia University, Montreal, Quebec, Canada
contact email	william.zerges@concordia.ca
lab head
Jamieson Dhaliwal
contact affiliation	Concordia University
contact email	jamieson.dhaliwal@concordia.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037184
     1. Label: PRIDE project
     2. Name: An RNA granule for translation quality control in Saccharomyces cerevisiae