PXD037175

PXD037175 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Glycoengineering of hCK and MDCK for influenza A virus propagation
Description	Recent human H3N2 influenza A viruses have evolved to bind elongated glycans terminated with α2,6-linked sialic acids. These extended glycans are of low abundance on cells that are used for the propagation of these viruses, which may explain why it is very difficult to isolate and propagate human H3N2 viruses. This greatly hampers the further study of these viruses. Therefore, we elongated the glycans on cells (MDCK and hCK) by overexpression of the glycosyltransferases that are responsible for building N-acetyllactosamine (LacNAc) repeats, namely B3GNT2 and B4GALT1. Overexpression was clearly shown by qPCR and MS experiments.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:46:08.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joost Snijder
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-04 02:41:18	ID requested
⏵ 1	2024-01-26 06:46:09	announced

Publication List

10.1093/glycob/cwad060;
Spruit CM, Sweet IR, Maliepaard JCL, Bestebroer T, Lexmond P, Qiu B, Damen MJA, Fouchier RAM, Reiding KR, Snijder J, Herfst S, Boons GJ, de Vries RP, Contemporary human H3N2 influenza A viruses require a low threshold of suitable glycan receptors for efficient infection. Glycobiology, 33(10):784-800(2023) [pubmed]

10.1093/glycob/cwad060;

Spruit CM, Sweet IR, Maliepaard JCL, Bestebroer T, Lexmond P, Qiu B, Damen MJA, Fouchier RAM, Reiding KR, Snijder J, Herfst S, Boons GJ, de Vries RP, Contemporary human H3N2 influenza A viruses require a low threshold of suitable glycan receptors for efficient infection. Glycobiology, 33(10):784-800(2023) [pubmed]

Keyword List

submitter keyword: influenza
glycosylation

submitter keyword: influenza

glycosylation

Contact List

Joost Snijder
contact affiliation	Utrecht University
contact email	j.snijder@uu.nl
lab head
Joost Snijder
contact affiliation	Utrecht University
contact email	joost.snijder@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD037175
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD037175

PRIDE project URI

Repository Record List

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