PXD037167

PXD037167 is an original dataset announced via ProteomeXchange.

Title	Molecular Acclimation of Halobacterium salinarum to Halite Brine Inclusions
Description	Halophilic microorganisms have long been known to survive within the brine inclusions of salt crystals, as evidenced by their pigmentation. However, the molecular mechanisms allowing this survival has remained an open question for decades. While protocols for the surface sterilization of halite (NaCl) have enabled isolation of cells and DNA from within halite brine inclusions, “-omics” based approaches have faced two main technical challenges: (1) removal of all contaminating organic biomolecules (including proteins) from halite surfaces, and (2) performing selective biomolecule extractions directly from cells contained within halite brine inclusions with sufficient speed to avoid modifications in gene expression during extraction. In this study, we present methods to resolve these two technical challenges. In addition, we apply these methods to perform the first examination of the early acclimation of a model haloarchaeon (Halobacterium salinarum NRC-1) to halite brine inclusions. Examinations of the proteome of Halobacterium cells two months post-evaporation revealed a high degree of similarity with stationary phase liquid cultures, but with a sharp down-regulation of ribosomal proteins. Low quantities of RNA from halite brine inclusions corroborate the hypothesis of low transcriptional and translational activities. While proteins for central metabolism were part of the shared proteome between liquid cultures and halite brine inclusions, proteins involved in cell mobility (archaellum, gas vesicles) were either absent or less abundant in halite samples. Proteins unique to cells within brine inclusions included transporters, suggesting modified interactions between cells and the surrounding brine inclusions microenvironment. The methods and hypotheses presented here enable future studies of the survival of halophiles in both culture model and natural halite systems.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:25:34.860.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037167
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Severine Zirah
SpeciesList	scientific name: Halobacterium salinarum; NCBI TaxID: 2242;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-03 11:40:54	ID requested
1	2023-03-10 15:02:00	announced
⏵ 2	2023-11-14 07:25:37	announced	2023-11-14: Updated project metadata.

10.6019/PXD037167;

Favreau C, Tribondeau A, Marugan M, Guyot F, Alpha-Bazin B, Marie A, Puppo R, Dufour T, Huguet A, Zirah S, Kish A, to halite brine inclusions. Front Microbiol, 13():1075274(2022) [pubmed]

submitter keyword: Halobacterium, halophile, halite

Philippe Grellier
contact affiliation	Museum national d'Histoire naturelle
contact email	philippe.grellier@mnhn.fr
lab head
Severine Zirah
contact affiliation	Museum national d'Histoire naturelle
contact email	severine.zirah@mnhn.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037167
     1. Label: PRIDE project
     2. Name: Molecular Acclimation of Halobacterium salinarum to Halite Brine Inclusions