PXD037165

PXD037165 is an original dataset announced via ProteomeXchange.

Title	Depletion Assisted Hemin Affinity (DAsHA) Proteomics Reveals an Expanded Landscape of Heme Binding Proteins in the Human Proteome
Description	Heme b (iron protoporphyrin IX) plays important roles in biology as a metallocofactor and signaling molecule. However, the targets of heme signaling and the network of proteins that mediate the exchange of heme from sites of synthesis or uptake to heme dependent or regulated proteins are poorly understood. Herein, we describe a quantitative mass spectrometry-based chemoproteomics strategy to identify exchange labile hemoproteins in human embryonic kidney HEK293 cells that may be relevant to heme signaling and trafficking. The strategy involves depleting endogenous heme with the heme biosynthetic inhibitor succinylacetone (SA), leaving putative heme binding proteins in their apo-state, followed by the capture of those proteins using hemin-agarose resin and finally elution and identification by mass spectrometry. By identifying only those proteins that interact with high specificity to hemin-agarose relative to control beaded agarose in a SA-dependent manner, we have expanded the number of proteins and ontologies that may be involved in binding and buffering labile heme or are targets of heme signaling. Notably, these include proteins involved in chromatin remodeling, DNA damage response, RNA splicing, cytoskeletal organization and vesicular trafficking, many of which have been associated with heme through complementary studies published recently. Taken together, these results provide support for the emerging role for heme in an expanded set of cellular processes from genome integrity to protein trafficking and beyond.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:10.787.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Torres
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-03 11:40:17	ID requested
1	2023-05-10 10:07:56	announced
⏵ 2	2023-11-14 08:46:20	announced	2023-11-14: Updated project metadata.

Kim H, Moore CM, Mestre-Fos S, Hanna DA, Williams LD, Reddi AR, Torres MP, Depletion assisted hemin affinity (DAsHA) proteomics reveals an expanded landscape of heme-binding proteins in the human proteome. Metallomics, 15(3):(2023) [pubmed]

submitter keyword: Heme, heme trafficking,Proteomics, heme binding protein, cell stress, heme affinity, hemin agarose, labile heme pool

Matthew Torres
contact affiliation	School of Biological Sciences, Georgia Institute of Technology
contact email	mtorres35@gatech.edu
lab head
Matthew Torres
contact affiliation	Georgia Institute of Technology
contact email	mtorres35@gatech.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037165
     1. Label: PRIDE project
     2. Name: Depletion Assisted Hemin Affinity (DAsHA) Proteomics Reveals an Expanded Landscape of Heme Binding Proteins in the Human Proteome