PXD037135

PXD037135 is an original dataset announced via ProteomeXchange.

Title	Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae — Spot Identifications
Description	Hexokinase 2 (Hxk2) of Saccharomyces cerevisiae is a dual function hexokinase, acting as a glycolytic enzyme and being involved in the transcriptional regulation of glucose-repressible genes. Relief from glucose repression is accompanied by phosphorylation of Hxk2 at serine 15, which has been attributed to the protein kinase Tda1. To explore the role of Tda1 beyond Hxk2 phosphorylation, the proteomic consequences of TDA1 deficiency were investigated by difference gel electrophoresis (2D-DIGE) comparing a wild type and a Δtda1 deletion mutant. To additionally address possible consequences of glucose repression/derepression, both were grown at 2 % and 0.1 % (w/v) glucose. A total of eight protein spots exhibiting a minimum twofold enhanced or reduced fluorescence upon TDA1 deficiency was detected and identified by mass spectrometry. Among the spot identities are – besides the expected Hxk2 – two proteoforms of hexokinase 1 (Hxk1). Targeted proteomics analyses in conjunction with 2D-DIGE demonstrated that TDA1 is indispensable for Hxk2 and Hxk1 phosphorylation at serine 15. Thirty-six glucose-concentration-dependent protein spots were identified. A simple method to improve spot quantification, approximating spots as rotationally symmetric solids, is presented along with new data on the quantities of Hxk1 and Hxk2 and their serine 15 phosphorylated forms at high and low glucose growth conditions. The Δtda1 deletion mutant exhibited no altered growth under high or low glucose conditions or on alternative carbon sources. Also, invertase activity, serving as a reporter for glucose derepression, was not significantly altered. Instead, an involvement of Tda1 in oxidative stress response is suggested.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:06.176.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henry Mueller
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	L-methionine sulfoxide
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-02 03:58:35	ID requested
1	2022-10-03 00:56:43	announced
⏵ 2	2023-11-14 08:58:06	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics, Hxk2, glucose signaling, Hxk1,serine/threonine protein kinase, yeast, hexokinase, glucose metabolism, phosphorylation, Ymr291w/Tda1

Marc Gentzel
contact affiliation	Molecular Analysis - Mass Spectrometry, Center for Molecular and Cellular Bioengineering (CMCB), TU Dresden
contact email	marc.gentzel@tu-dresden.de
lab head
Henry Mueller
contact affiliation	Institute of Physiological Chemistry, Medizinische Fakultät Carl Gustav Carus, TU Dresden
contact email	henry.mueller2@tu-dresden.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD037135

PRIDE project URI

• PRIDE
  1. PXD037135
     1. Label: PRIDE project
     2. Name: Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae — Spot Identifications