PXD037117

PXD037117 is an original dataset announced via ProteomeXchange.

Title	TORC1 phosphorylates and inhibits the ribosome preservation factor Stm1 to activate dormant ribosomes
Description	Target of rapamycin complex 1 (TORC1) promotes biogenesis and inhibits degradation of ribosomes in response to nutrient availability. To ensure a basal supply of ribosomes, cells preserve a small pool of dormant ribosomes under nutrient-limited conditions. The regulation of dormant ribosomes is poorly characterized. Here, we show that upon inhibition of TORC1 by rapamycin or nitrogen starvation, Stm1 (suppressor of target of Myb protein 1) forms non-translating, dormant 80S ribosomes. Furthermore, Stm1-bound 80S ribosomes are protected from proteasomal degradation. Upon re-feeding, TORC1 directly phosphorylates and inhibits Stm1, thereby reactivating translation. Finally, SERBP1 (SERPINE1 mRNA binding protein), a mammalian ortholog of Stm1, forms dormant 80S ribosomes upon mTORC1 inhibition in mammalian cells. Thus, TORC1 regulates ribosomal dormancy in an evolutionarily conserved manner via a ribosome preservation factor.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:21.800.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037117
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Danilo Ritz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-30 04:10:27	ID requested
1	2023-01-19 03:04:51	announced
⏵ 2	2023-11-14 08:58:22	announced	2023-11-14: Updated project metadata.

10.6019/PXD037117;

submitter keyword: Protein Synthesis, Dormant Ribosome, Proteasome, TORC1, Ribosome preservation Factor, Ribophagy

Alexander Schmidt
contact affiliation	Proteomics Core Facility Biozentrum, Universität Basel Spitalstrasse 41 CH - 4056 Basel Schweiz
contact email	alex.schmidt@unibas.ch
lab head
Danilo Ritz
contact affiliation	Proteomics Core Facility Biozentrum, University of Basel Klingelbergstrasse 50/70 CH-4056 Basel / Switzerland
contact email	danilo.ritz@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037117
     1. Label: PRIDE project
     2. Name: TORC1 phosphorylates and inhibits the ribosome preservation factor Stm1 to activate dormant ribosomes