PXD037114

PXD037114 is an original dataset announced via ProteomeXchange.

Title	A chemoproteomic platform for selective deubiquitinase inhibitor discovery
Description	Here we describe our work on the development of an ABPP screening platform for cysteine reactive covalent fragments against deubiquitinating enzymes (DUBs) on 96 well plate format. Within this targeted workflow, we profile cysteine reactive fragments by competition with biotinylated ubiquitin probe in cell lysates and employ label free data independent acquisition (DIA) MS method. The platform was used to screen a library of 138 Cys-reactive covalent fragments against DUBome in HEK293T cell lysate, identifying functionally relevant hit fragments for numerous DUBs and demonstrating the utility of the approach in expanding the liganded proteome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:33:57.056.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aini Vuorinen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-30 04:09:38	ID requested
1	2024-03-18 03:55:02	announced
⏵ 2	2024-10-22 06:33:57	announced	2024-10-22: Updated project metadata.

10.1016/J.XCRP.2023.101636;

submitter keyword: DUBs,covalent probes, covalent fragment screening, chemical biology, activity-based protein profiling

Jacob Bush
contact affiliation	GSK
contact email	jacob.x.bush@gsk.com
lab head
Aini Vuorinen
contact affiliation	GSK
contact email	aini.e.vuorinen@gsk.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD037114

PRIDE project URI

• PRIDE
  1. PXD037114
     1. Label: PRIDE project
     2. Name: A chemoproteomic platform for selective deubiquitinase inhibitor discovery