PXD037103

PXD037103 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicles from Aureobasidium pullulans
Description	Extracellular vesicles (EVs) are increasingly recognized as an important mechanism for cell-cell interactions. Their role in fungi is still poorly understood and they have been isolated from only a handful of species. Here, we isolated and characterized EVs from Aureobasidium pullulans, a biotechnologically important black yeast-like fungus that is increasingly used for biocontrol of phytopathogenic fungi and bacteria. After optimization of the isolation protocol, characterization of EVs from A. pullulans by transmission electron microscopy (TEM) revealed a typical cup-shaped morphology and different subpopulations of EVs. These results were confirmed by nanoparticle tracking analysis (NTA), which revealed that A. pullulans produced 6.1 × 10^8 nanoparticles per milliliter of culture medium. Proteomic analysis of EVs detected 642 proteins. A small fraction of them had signal peptides for secretion and transmembrane domains. Proteins characteristic of different synthesis pathways were found, suggesting that EVs are synthesized by multiple pathways in A. pullulans. Enrichment analysis using Gene Ontology showed that most of the proteins found in the EVs were associated with primary metabolism. When sequencing the small RNA fraction of A. pullulans EVs, we found two hypothetical novel mil-RNAs. Finally, we tested the biocontrol potential of EVs from A. pullulans. The EVs did not inhibit the germination of spores of three important phytopathogenic fungi – Botrytis cinerea, Colletotrichum acutatum, and Penicillium expansum. However, exposure of grown cultures of C. acutatum and P. expansum to A. pullulans EVs resulted in visible changes in morphology of colonies. These preliminary results suggest that EVs may be part of the antagonistic activity of A. pullulans, which is so far only partially understood. Thus, the first isolation and characterization of EVs from A. pullulans provides a starting point for further studies of EVs in the biotechnologically important traits of the biocontrol black fungus A. pullulans in particular and in the biological role of fungal EVs in general.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:27:34.107.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anja Černoša
SpeciesList	scientific name: Aureobasidium pullulans EXF-150; NCBI TaxID: 1043002;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-30 04:02:04	ID requested
1	2022-11-09 03:08:59	announced
⏵ 2	2023-11-14 08:27:39	announced	2023-11-14: Updated project metadata.

Č, erno, š, a A, Gostin, č, ar C, Lavrin T, Kostanj, š, ek R, Lenassi M, Gunde-Cimerman N, Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans. Fungal Biol Biotechnol, 9(1):16(2022) [pubmed]

submitter keyword: extracellular vesicles, Aureobasidium pullulans, fungi

Nina Gunde-Cimerman
contact affiliation	Department of Biology, Chair of Molecular Genetics and Microbiology, University of Ljubljana, Slovenia
contact email	Nina.Gunde-Cimerman@bf.uni-lj.si
lab head
Anja Černoša
contact affiliation	Young Researcher
contact email	anja.cernosa@bf.uni-lj.si
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD037103

PRIDE project URI

• PRIDE
  1. PXD037103
     1. Label: PRIDE project
     2. Name: Extracellular vesicles from Aureobasidium pullulans