PXD037091

PXD037091 is an original dataset announced via ProteomeXchange.

Title	Protein interaction partners of FRUITFULL in inflorescence meristems and pistils by IP-MS.
Description	To study the possibility that the tissue-specific gene targets and regulation by FUL are due to a different composition in the FUL transcription factor (TF) protein complex/es in both tissues, we determined the protein-protein interactions of FUL in planta. Inflorescence meristems (IMs) of 35S:AP1-GR ap1 cal pFUL:FUL-GFP plants were collected to identify meristem-specific protein complexes of FUL, and stage 12 - 16 pistils of pFUL:FUL-GFP ful-1 plants were collected for the pistil-specific FUL protein complexes. Protein complexes were isolated by immunoprecipitation (IP) using anti-GFP antibodies and protein identification was performed using LC-MS/MS, followed by label-free protein quantification.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:34.363.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sjef Boeren
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-30 03:46:56	ID requested
⏵ 1	2023-11-14 08:44:42	announced

van Mourik H, Chen P, Smaczniak C, Boeren S, Kaufmann K, Bemer M, Angenent GC, Muino JM, Dual specificity and target gene selection by the MADS-domain protein FRUITFULL. Nat Plants, 9(3):473-485(2023) [pubmed]

submitter keyword: Arabidopsis thaliana, IP-MS, protein complex, FRUITFULL

Sjef Boeren
contact affiliation	Wageningen University
contact email	sjef.boeren@wur.nl
lab head
Sjef Boeren
contact affiliation	Biochemistry
contact email	sjef.boeren@wur.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037091
     1. Label: PRIDE project
     2. Name: Protein interaction partners of FRUITFULL in inflorescence meristems and pistils by IP-MS.