PXD037090

PXD037090 is an original dataset announced via ProteomeXchange.

Title	Smg6 interacting partners in mouse
Description	Nonsense-mediated RNA decay (NMD) is a highly conserved and selective RNA turnover pathway that depends on the endonuclease SMG6. Here, we show that SMG6 is essential for male germ cell differentiation in mice. Germ-cell conditional knockout (cKO) of Smg6 induces extensive transcriptome misregulation, including a failure to eliminate meiotically expressed transcripts in early haploid cells, and accumulation of NMD target mRNAs with long 3’ untranslated regions (UTRs). Loss of SMG6 in the male germline results in complete arrest of spermatogenesis at the early haploid cell stage. We find that SMG6 is strikingly enriched in the chromatoid body (CB), a specialized cytoplasmic granule in male germ cells also harboring PIWI-interacting RNAs (piRNAs) and the piRNA-binding protein PIWIL1.
HostingRepository	PRIDE
AnnounceDate	2022-10-01
AnnouncementXML	Submission_2022-09-30_21:04:30.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lin Ma
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-30 03:46:40	ID requested
⏵ 1	2022-09-30 21:04:31	announced

Dataset with its publication pending

submitter keyword: Smg6, Smg7, chromatoid body, mouse

Noora Kotaja
contact affiliation	Institute of Biomedicine, University of Turku, Finland
contact email	nookot@utu.fi
lab head
Lin Ma
contact affiliation	University of Turku
contact email	malin.ml@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD037090

PRIDE project URI

• PRIDE
  1. PXD037090
     1. Label: PRIDE project
     2. Name: Smg6 interacting partners in mouse