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PXD037068

PXD037068 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis
DescriptionThe coronavirus disease 2019 (COVID-19) pandemic has devastated global health. Identifying key host factors essential for SARS-CoV-2 RNA replication is expected to unravel cellular targets for the development of broad-spectrum antiviral drugs which have been quested for the preparedness of future viral outbreaks. Here, we have identified host proteins that associate with non-structural protein 12 (nsp12), the RNA-dependent RNA polymerase (RdRp) of SARS-CoV-2 using a mass spectrometry (MS)-based proteomic approach. Among the candidate factors, CDK2 (Cyclin-dependent kinase 2), a member of cyclin-dependent kinases, interacts with nsp12 and causes its phosphorylation at T20, thus facilitating the assembly of the RdRp complex consisting of nsp12, nsp7 and nsp8 and promoting efficient synthesis of viral RNA. The crucial role of CDK2 in viral RdRp function is further supported by our observation that CDK2 inhibitors potently impair viral RNA synthesis and SARS-CoV-2 infection. Taken together, we have discovered CDK2 as a key host factor of SARS-CoV-2 RdRp complex, thus serving a promising target for the development of SARS-CoV-2 RdRp inhibitors.
HostingRepositoryiProX
AnnounceDate2022-09-28
AnnouncementXMLSubmission_2023-03-28_20:34:29.602.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSaisai Guo
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive Plus; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-28 19:25:13ID requested
12023-03-27 19:42:35announced
22023-03-28 20:34:30announced2023-03-29: Update publication information.
Publication List
Guo S, Lei X, Chang Y, Zhao J, Wang J, Dong X, Liu Q, Zhang Z, Wang L, Yi D, Ma L, Li Q, Zhang Y, Ding J, Liang C, Li X, Guo F, Wang J, Cen S, SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis. Signal Transduct Target Ther, 7(1):400(2022) [pubmed]
Keyword List
submitter keyword: SARS-CoV-2, RdRp, CDK, phosphorylation, nsp12
Contact List
Shan Cen
contact affiliationInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School
contact emailshancen@imb.pumc.edu.cn
lab head
Saisai Guo
contact affiliationInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School
contact emailgss2029@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI