PXD037068
PXD037068 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis |
Description | The coronavirus disease 2019 (COVID-19) pandemic has devastated global health. Identifying key host factors essential for SARS-CoV-2 RNA replication is expected to unravel cellular targets for the development of broad-spectrum antiviral drugs which have been quested for the preparedness of future viral outbreaks. Here, we have identified host proteins that associate with non-structural protein 12 (nsp12), the RNA-dependent RNA polymerase (RdRp) of SARS-CoV-2 using a mass spectrometry (MS)-based proteomic approach. Among the candidate factors, CDK2 (Cyclin-dependent kinase 2), a member of cyclin-dependent kinases, interacts with nsp12 and causes its phosphorylation at T20, thus facilitating the assembly of the RdRp complex consisting of nsp12, nsp7 and nsp8 and promoting efficient synthesis of viral RNA. The crucial role of CDK2 in viral RdRp function is further supported by our observation that CDK2 inhibitors potently impair viral RNA synthesis and SARS-CoV-2 infection. Taken together, we have discovered CDK2 as a key host factor of SARS-CoV-2 RdRp complex, thus serving a promising target for the development of SARS-CoV-2 RdRp inhibitors. |
HostingRepository | iProX |
AnnounceDate | 2022-09-28 |
AnnouncementXML | Submission_2023-03-28_20:34:29.602.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Saisai Guo |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive Plus; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-09-28 19:25:13 | ID requested | |
1 | 2023-03-27 19:42:35 | announced | |
⏵ 2 | 2023-03-28 20:34:30 | announced | 2023-03-29: Update publication information. |
Publication List
Guo S, Lei X, Chang Y, Zhao J, Wang J, Dong X, Liu Q, Zhang Z, Wang L, Yi D, Ma L, Li Q, Zhang Y, Ding J, Liang C, Li X, Guo F, Wang J, Cen S, SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis. Signal Transduct Target Ther, 7(1):400(2022) [pubmed] |
Keyword List
submitter keyword: SARS-CoV-2, RdRp, CDK, phosphorylation, nsp12 |
Contact List
Shan Cen | |
---|---|
contact affiliation | Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School |
contact email | shancen@imb.pumc.edu.cn |
lab head | |
Saisai Guo | |
contact affiliation | Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School |
contact email | gss2029@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |