PXD037047 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Peptidomic analysis of bacterial protease activity in acute wound fluids |
Description | The clinical assessment of wound infection and related complications is challenging and the development of objective methods to measure wound status and predict healing outcomes is therefore essential. As bacterial protease activities play important roles in infection, analysis of changes in the wound peptidome by individual bacterial enzymes could provide insight into proteolytic events occurring in infected wounds, and may aid in the discovery of biomarkers. Wound infection was mimicked by incubating sterile acute wound fluids ex vivo with the bacterial proteases Pseudomonas aeruginosa LasB and Staphyloccocus aureus V8. Using a peptidomics approach, we characterized the low-molecular-weight peptidome of the digested samples and identified over 100 protein targets for each enzyme. Additionally, we found enzyme-specific peptides and digestion patterns. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:32:24.153.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mariena van der Plas |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deamidated residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-27 11:40:09 | ID requested | |
1 | 2024-03-07 01:44:29 | announced | |
⏵ 2 | 2024-10-22 06:32:25 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.isci.2024.109005; |
Cai J, Nielsen MW, Kalogeropoulos K, Auf dem Keller U, van der Plas MJA, Peptidomic analysis of endogenous and bacterial protease activity in human plasma and wound fluids. iScience, 27(2):109005(2024) [pubmed] |
Keyword List
submitter keyword: Human, LC-MSMS, wound fluids proteases, peptidome |
Contact List
Mariena J.A. |
contact affiliation | LEO Foundation Center for Cutaneous Drug Delivery Department of Pharmacy University of Copenhagen Denmark |
contact email | mariena.van_der_plas@sund.ku.dk |
lab head | |
Mariena van der Plas |
contact affiliation | University of Copenhagen Department of Pharmacy |
contact email | mariena.van_der_plas@sund.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037047
- Label: PRIDE project
- Name: Peptidomic analysis of bacterial protease activity in acute wound fluids