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PXD037047

PXD037047 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePeptidomic analysis of bacterial protease activity in acute wound fluids
DescriptionThe clinical assessment of wound infection and related complications is challenging and the development of objective methods to measure wound status and predict healing outcomes is therefore essential. As bacterial protease activities play important roles in infection, analysis of changes in the wound peptidome by individual bacterial enzymes could provide insight into proteolytic events occurring in infected wounds, and may aid in the discovery of biomarkers. Wound infection was mimicked by incubating sterile acute wound fluids ex vivo with the bacterial proteases Pseudomonas aeruginosa LasB and Staphyloccocus aureus V8. Using a peptidomics approach, we characterized the low-molecular-weight peptidome of the digested samples and identified over 100 protein targets for each enzyme. Additionally, we found enzyme-specific peptides and digestion patterns.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:32:24.153.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMariena van der Plas
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-27 11:40:09ID requested
12024-03-07 01:44:29announced
22024-10-22 06:32:25announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.isci.2024.109005;
Cai J, Nielsen MW, Kalogeropoulos K, Auf dem Keller U, van der Plas MJA, Peptidomic analysis of endogenous and bacterial protease activity in human plasma and wound fluids. iScience, 27(2):109005(2024) [pubmed]
Keyword List
submitter keyword: Human, LC-MSMS, wound fluids proteases, peptidome
Contact List
Mariena J.A.
contact affiliationLEO Foundation Center for Cutaneous Drug Delivery Department of Pharmacy University of Copenhagen Denmark
contact emailmariena.van_der_plas@sund.ku.dk
lab head
Mariena van der Plas
contact affiliationUniversity of Copenhagen Department of Pharmacy
contact emailmariena.van_der_plas@sund.ku.dk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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