PXD037043

PXD037043 is an original dataset announced via ProteomeXchange.

Title	McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylation
Description	The centriole duplication cycle must be tightly controlled and coordinated with the chromosome cycle to maintain a proper number of centrioles in cells. Aberrations in centriole biogenesis can lead to cancer, developmental disorders and ciliopathies, yet the molecular determinants of centriole number control and the link between the centrosome and chromosome cycles remain poorly characterized. Here, we show that McIdas -previously implicated in cell cycle control and centriole amplification in multiciliated cells- is critical to maintain a proper number of centrioles in proliferating cells. Using expansion microscopy, we demonstrate that McIdas is present at the middle part of centrioles, where it exhibits a differential localization from the mother to the daughter centriole as the cell cycle proceeds. Regulation of McIdas expression is critical for the maintenance of correct centriole number in normal and cancer cells. Loss of McIdas perturbs daughter centriole biogenesis and centrosomal SAS6 recruitment, whereas its ectopic overexpression induces centriole overduplication. Consistently, McIdas depletion reduces PLK4-induced centriole amplification. We further show that McIdas interacts with PLK4 and is a novel substrate of this kinase. Multiple PLK4-specific phosphorylation sites on McIdas protein were identified through tandem mass-spectrometry analysis and characterized as important for McIdas role on centriole duplication. Our results demonstrate that McIdas is localized at centrosomes and affects the core centriole duplication machinery. This novel, direct role of McIdas on centriole duplication could link its previously seemingly unrelated functions in the chromosome cycle and multiciliogenesis.
HostingRepository	PRIDE
AnnounceDate	2025-12-02
AnnouncementXML	Submission_2025-12-02_01:49:15.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-27 11:36:39	ID requested
⏵ 1	2025-12-02 01:49:16	announced

Dataset with its publication pending

submitter keyword: McIdas, proteomics, phosphorylation, cell cycle,centrosome, PLK4

Matthias Mann
contact affiliation	Director,Max Planck Institute of Biochemistry
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037043
     1. Label: PRIDE project
     2. Name: McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylation